1887

Abstract

The fungicide Captan inhibited the growth of at a concentration of 25 μg ml. When cysteine and glutathione were added to the medium they removed the toxicity of the fungicide. A spontaneous mutant was isolated which was able to grow and fix nitrogen in the presence of 100 μg Captan ml. Characterization of the mutant indicated very high levels of glutathione and glutathione transferase activity as compared to the parent strain. The role of these cellular components in the mechanism of resistance to Captan is discussed; the involvement of a selenium-independent glutathione peroxidase appears essential to Captan resistance in the mutant strain.

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1988-12-01
2021-08-01
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References

  1. Agnihotri V.P. 1971; Persistence of captan and its effect on microflora, respiration and nitrification of forest nursery soil.. Canadian Journal of Microbiology 17:377–383
    [Google Scholar]
  2. Akerboom T.P.M., Sies H. 1981; Assay of glutathione, glutathione disulfide, and glutathione mixed disulfides in biological samples.. Methods in Enzymology 77:373–382
    [Google Scholar]
  3. Alvarez R., Sleiman L.A. 1983; Effects of some pesticides on Azospirillum lipoferum and Azospirillum brasilense in pure culture.. Revista Facultad de Agronomia 4:277–281
    [Google Scholar]
  4. Anderson J.R. 1978; Pesticide effects on non-target soil microorganisms.. In Pesticide Microbiology pp. 313–533 Hill I.R., Wright S.J.L. Edited by London: Academic Press;
    [Google Scholar]
  5. Banerjee A., Banerjee A.K. 1987; Influence of captan on some microorganisms and microbial processes related to the nitrogen cycle.. Plant and Soil 102:239–245
    [Google Scholar]
  6. Bani D., Barberio C., Bazzicalupo M., Favilli F., Gallori E., Polsinelli M. 1980; Isolation and characterization of glutamate synthase mutants of Azospirillum brasilense.. Journal of General Microbiology 119:239–244
    [Google Scholar]
  7. Carlberg I., Mannervik B. 1975; Purification and characterization of the flavoenzyme glutathione reductase from rat liver.. Journal of Biological Chemistry 250:5475–5480
    [Google Scholar]
  8. Curley R.L., Burton J.C. 1975; Compatibility of Rhizobium japonicum with chemical seed protectants.. Agronomy Journal 67:807–808
    [Google Scholar]
  9. Döbereiner J., Day J.M. 1975; Associative symbiosis in tropical grasses: characterization of microorganisms and dinitrogen fixing sites.. In Proceedings of the 1st International Symposium on Nitrogen Fixation pp. 518–538 Newton W.E., Nyman J.C. Edited by Pullman: Washington State University Press;
    [Google Scholar]
  10. Fahey R.C., Brown W.C., Adams W.B., Worsham M.B. 1978; Occurrence of glutathione in bacteria.. Journal of Bacteriology 133:1126–1129
    [Google Scholar]
  11. Gadkari D. 1988; Influence of herbicides on growth and nitrogenase activity of Azospirillum . In Azospirillum. IV Genetics, Physiology, Ecology pp. 150–158 Klingmuller W. Edited by Berlin: Springer;
    [Google Scholar]
  12. Gallori E., Bazzicalupo M. 1985; Effect of nitrogen compounds on nitrogenase activity in Azospirillum brasilense . FEMS Microbiology Letters 28:35–38
    [Google Scholar]
  13. Graham P.H., Ocampo G., Ruiz L.D., Duque A. 1980; Survival of Rhizobium phaseoli in contact with chemical seed protectants. Agronomy Journal 72:625–627
    [Google Scholar]
  14. Habig V.H., Pabst M.J., Jakoby W.B. 1974; Glutathione S-transferases, the first enzymatic step in mercapturic acid formation. Journal of Biological Chemistry 249:7130–7139
    [Google Scholar]
  15. Lawerence R.A., Burk R.F. 1976; Glutathione peroxidase activity in selenium deficient rat liver. Biochemical and Biophysical Research Communications 71:952–958
    [Google Scholar]
  16. Lawerence R.A., Parkhill L.K., Burk R.F. 1978; Hepatic cytosolic non selenium-dependent glutathione peroxidase activity: its nature and the effect of the selenium deficiency. Journal of Nutrition 108:981–987
    [Google Scholar]
  17. Odeyemi O., Alexander M. 1977; Resistance of Rhizobium strains to phygon, spergon and thiuram. Applied and Environmental Microbiology 133:784–790
    [Google Scholar]
  18. Okon Y. 1985a; The physiology of Azospirillum in relation to its utilization as inoculum for promoting growth of plants. In N2-fixation and C02 Metabolism pp. 165–174 Ludden P.W., Bums S.B. Edited by New York: Elsevier;
    [Google Scholar]
  19. Okon Y. 1985b; Azospirillum as a potential inoculant for agriculture. Trends in Biotechnology 3:223–228
    [Google Scholar]
  20. Okon Y., Kapulnik Y. 1986; Development and function of Azospirillum inoculated roots. Plant and Soil 90:3–16
    [Google Scholar]
  21. Postgate J.R. 1972; The acetylene reduction test for nitrogen fixation. Methods in Microbiology 6B:343–365
    [Google Scholar]
  22. Prohaska J.R. 1980; The glutathione peroxidase activity of glutathione S-transferase. Biochimica et biophysica acta 611:87–98
    [Google Scholar]
  23. Rennie R.J. 1986; Selection for captan tolerance in the Rhizobium phaseoli-Phaseolus vulgaris L. N2- fixing symbiosis. Canadian Journal of Soil Science 66:143–150
    [Google Scholar]
  24. Rennie R.J., Dubetz S. 1984; Effect of fungicide and herbicide on nodulation and N2 fixation in soybean fields lacking indigenous Rhizobium japonicum . Agronomy Journal 76:451–454
    [Google Scholar]
  25. Rennie R.J., Howard R.J., Swanson T.A., Flores G.H.A. 1985; The effect of seed-applied pesticides on growth and N2 fixation in pea, lentil and fababean. Canadian Journal of Plant Science 65:23–28
    [Google Scholar]
  26. Ruiz-Sainz J.E., Beringer J.E., Gutierrez-Navarro A.M. 1984; Effect of the fungicide captafol on the survival and symbiotic properties of Rhizobium trifolii . Journal of Applied Bacteriology 57:361–367
    [Google Scholar]
  27. Ruplal Saxena D.M. 1980; Cytological and biochemical effects of pesticides on microorganisms. Residue Review 73:49–86
    [Google Scholar]
  28. Simon-Sylvestre G., Fournier J.C. 1979; Effects of pesticides on the soil microflora. Advances in Agronomy 31:1–92
    [Google Scholar]
  29. Sisler H.D. 1982; Biodegradation of agricultural fungicides. In Biodegradation of Pesticides pp. 133–155 Matsamura F., Krishna Marti C.R. Edited by New York: Plenum Press;
    [Google Scholar]
  30. Somerville L. 1986; The metabolism of fungicides. Xenobiotica 16:1017–1030
    [Google Scholar]
  31. Tappel M.E., Chaudier J. 1982; Glutathione peroxidase activities of animal tissues. Comparative Biochemistry and Physiology 73B:945–949
    [Google Scholar]
  32. Wendel A. 1980; Glutathione peroxidase. In Enzymatic Basis of Detoxification 769 pp. 333–348 Jakoby W.B. Edited by New York: Academic Press;
    [Google Scholar]
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