1887

Abstract

K12 mutants lacking phenazine-methosulphate-linked formate dehydrogenase (FDH-PMS) activity, but still capable of producing normal levels of benzyl-viologen-linked formate dehydrogenase (FDH-BV) and nitrate reductase activities, have been isolated following P1 localized mutagenesis. The relevant mutations mapped with the same cotransduction frequency close to the gene, at 88 min on the chromosome. They were further subdivided into two classes. Class I consisted of six mutants which synthesized an inactive FDH-PMS protein with the same subunit composition as the wild-type enzyme. In contrast, class II contained four mutants totally devoid of this antigen. Construction of merodiploid strains harbouring various combinations of the mutated alleles, on the episome and on the chromosome, led to the restoration of FDH-PMS activity by complementation of the products encoded by the respective wild-type alleles. Difference spectroscopy suggested that both and mutants contained normal amounts of the cytochrome associated with FDH-PMS although the cytochrome had lost its capacity for formate-dependent reduction.

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/content/journal/micro/10.1099/00221287-134-12-3129
1988-12-01
2022-01-28
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References

  1. Azoulay E., Marty B. 1970; Etude du système muhienzymatique hydrogène lyase chez Escherichia coli K12 et ses mutants chlorate-rèsistants. European Journal of Biochemistry 13:168–173
    [Google Scholar]
  2. Barrett E.L., Riggs D.L. 1982; Salmonella typhimurium mutants defective in the formate dehydrogenase linked to nitrate reductase. Journal of Bacteriology 149:554–560
    [Google Scholar]
  3. Barrett E.L., Jackson C.E., Fukumoto H.T., Chang G.W. 1979; Formate dehydrogenase mutants of Salmonella typhimurium: a new medium for their isolation and new mutant classes. Molecular and General Genetics 177:95–101
    [Google Scholar]
  4. Cheppaux M., Pascal M.C., Cassb F. 1977; Formate hydrogenlyase system in Salmonella typhimurium LT2. European Journal of Biochemistry 72:149–155
    [Google Scholar]
  5. Cox J.C., Edwards E.S., Demoss J.A. 1981; Resolution of distinct selenium containing formate dehydrogenases from Escherichia coli . Journal of Bacteriology 145:1317–1324
    [Google Scholar]
  6. Denis M., Richaud P. 1982; Dynamics of COrecombination to fully reduced cytochrome c oxidase in plant mitochondria after low to flash photolysis. Biochemical Journal 206:379–385
    [Google Scholar]
  7. Enoch H.G., Lester R.L. 1974; The role of a novel 6-containing nitrate reductase and quinone in the in vitro reconstruction of formate-nitrate reductase activity of Escherichia coli . Biochemical and Biophysical Research Communications 61:1234–1241
    [Google Scholar]
  8. Enoch H.G., Lester R.L. 1975; Purification and properties of formate dehydrogenase and nitrate reductase from Escherichia coli . Journal of Biological Chemistry 250:6693–6705
    [Google Scholar]
  9. Giordano G., Graham A., Boxer D.H., Azoulay E. 1978; Characterization of the membrane-bound nitrate reductase activity of aerobically grown chlorate-sensitive mutants of Escherichia coli K12. FEBS Letters 95:290–294
    [Google Scholar]
  10. Giordano G., Haddock B.A., Boxer D.H. 1980; Molybdenum-limited growth achieved either phenotypically or genotypically and its effect on the synthesis of formate dehydrogenase and nitrate reductase by Escherichia coli K12. FEMS Microbiology Letters 8:229–235
    [Google Scholar]
  11. Giordano G., Medani C.L., Mandrand-Berthelot M.A., Boxer D.H. 1983; Formate dehydrogenases from Escherichia coli . FEMS Microbiology Letters 17:171–177
    [Google Scholar]
  12. Glaser J.H., Demoss J.A. 1972; Comparison of nitrate reductase mutants of Escherichia coli selected by alternative procedures. Molecular and General Genetics 116:1–10
    [Google Scholar]
  13. Graham A., Jenkins H.E., Smith N.H., Mandrand-Berthelot M.A., Haddock B.A., Boxer D.H. 1980; Synthesis of formate dehydrogenase and nitrate reductase proteins in various fdh and chi mutants of Escherichia coli . FEMS Microbiology Letters 7:145–151
    [Google Scholar]
  14. Hackett N.R., Bragg P.D. 1983a; Membrane cytochromes of Escherichia coli grown aerobically and anaerobically with nitrate. Journal of Bacteriology 154:708–718
    [Google Scholar]
  15. Hackbtt N.R., Bragg P.D. 1983b; Membrane cytochromes of Escherichia coli chi mutants. Journal of Bacteriolology 154:719–727
    [Google Scholar]
  16. Haddock B.A., Jones C.W. 1977; Bacterial respiration. Bacteriological Reviews 41:47–99
    [Google Scholar]
  17. Haddock B.A., Mandrand-Berthelot M.A. 1982; Escherichia coli formate-to-nitrate respiratory chain: genetic analysis. Biochemical Society Transactions 10:478–480
    [Google Scholar]
  18. Jones R.W., Garland P.B. 1977; Sites and specificity of the reaction of bipyridylium compounds with anaerobic respiratory enzymes of Escherichia coli . Biochemical Journal 164:199–211
    [Google Scholar]
  19. Lester R.L., Demoss J.A. 1971; Effects of molybdenum and selenite on formate and nitrate metabolism in Escherichia coli . Journal of Bacteriology 105:1006–1014
    [Google Scholar]
  20. Low K.B. 1972; Escherichia coli K-12 F-prime factors, old and new. Bacteriological Reviews 36:587–607
    [Google Scholar]
  21. Mandrand-Berthelot M.A., Wee M.Y.K., Haddock B.A. 1978; An improved method for the identification and characterization of mutants of Escherichia coli deficient in formate dehydrogenase activity. FEMS Microbiology Letters 4:37–40
    [Google Scholar]
  22. Miller J.H. 1972 Experiments in Molecular Genetics Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  23. Murgola E.J., Yanofsky C. 1974; Structural interactions between amino acid residues at positions 22 and 211 in the tryptophan synthetase alpha chain of E. coli . Journal of Bacteriology 117:444–448
    [Google Scholar]
  24. Peck H.D., Gest H. 1957; Formic dehydrogenase and the hydrogenlyase enzyme complex in the coliaerogenes group. Journal of Bacteriology 73:706–721
    [Google Scholar]
  25. Power J. 1967; The l-rhamnose genetic system in Escherichia coli K-12. Genetics 55:557–568
    [Google Scholar]
  26. Ruiz-Herrbra J., Demoss J.A. 1969; Nitrate reductase complex of Escherichia coli K12: participation of specific formate dehydrogenase and cytochrome b1 components in nitrate reduction. Journal of Bacteriology 99:720–729
    [Google Scholar]
  27. Stewart V., Macgregor C.H. 1982; Nitrate reductase in Escherichia coli K-12: involvement of chlC, chlE and chlG loci. Journal of Bacteriology 151:788–799
    [Google Scholar]
  28. Wu L.F., Mandrand-Berthelot M.A. 1986; Genetic and physiological characterization of new Escherichia cdli mutants impaired in hydrogenase activity. Biochimie 68:167–179
    [Google Scholar]
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