1887

Abstract

The results of a physiological study of the interaction between NHCl, inosine, and the stereoisomers of alanine during germination of spores of T are presented. Detailed kinetics for the germination of unheated spores in moderate concentrations of -alanine (in the absence of auto-inhibition due to alanine racemase) are established, as is the specificity of the stimulatory effect of NHCl in relation to other salts, amines, and germinants. The results suggest that NHCl and inosine affect an early step in germination closely related to the function of an -alanine receptor.

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1988-11-01
2021-07-23
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References

  1. Gould G. W. 1969; Germination. In The Bacterial Spore pp. 397–444 Gould G. W., Hurst A. Edited by London: Academic Press;
    [Google Scholar]
  2. Halmann M., Keynan A. 1962; Stages in germination of spores of Bacillus licheniformis . Journal of Bacteriology 84:1187–1193
    [Google Scholar]
  3. Hedblom M. L., Adler J. 1983; Chemotactic response of Escherichia coli to chemically synthesized amino acids. Journal of Bacteriology 155:1463–1466
    [Google Scholar]
  4. Hills G. M. 1949; Chemical factors in the germination of spore-bearing aerobes. The effects of amino acids on the germination of Bacillus anthracis, with observations on the relations of optical form to biological activity. Biochemical Journal 45:363–370
    [Google Scholar]
  5. Hills G. M. 1950; Chemical factors in the germination of spore-bearing aerobes: observations on the influence of species, strain and conditions of growth. Journal of General Microbiology 4:38–47
    [Google Scholar]
  6. Krask B. J. 1961; Discussion. In Spores II pp. Halvorson H. O. Edited by Minneapolis: Burgess Publishing Co;
    [Google Scholar]
  7. Lewis J. C. 1969; Dormancy. In The Bacterial Spore pp. 301–358 Gould G. W., Hurst A. Edited by London: Academic Press;
    [Google Scholar]
  8. Preston R. A., Douthit H. A. 1984a; Stimulation of germination of unactivated Bacillus cereus spores by ammonia. Journal of General Microbiology 130:1041–1050
    [Google Scholar]
  9. Preston R. A., Douthit H. A. 1984b; Germination of Bacillus cereus spores: critical control by DL- alanine racemase. Journal of General Microbiology 130:3123–3133
    [Google Scholar]
  10. Preston R. A., Douthit H. A. 1984c; Adventitious racemization of L-alanine in studies of Bacillus spore germination. Spore Newsletter 8:142–145
    [Google Scholar]
  11. Segel I. H. 1975 Enzyme Kinetics p. 362. New York: John Wiley;
    [Google Scholar]
  12. Stewart G.S.A.B., Johnstone K., Hagelberg E., Ellar D. J. 1981; Commitment of bacterial spores to germinate. Biochemical Journal 198:101–106
    [Google Scholar]
  13. Warren S. C., Gould G. W. 1968; Bacillus cereus spore germination: absolute requirement for an amino acid. Biochimica et biophysica acta 170:341–350
    [Google Scholar]
  14. Woese C. R., Vary J. C., Halvorson H. O. 1968; A kinetic model for bacterial spore germination. Proceedings of the National Academy of Sciences of the United States of America 59:869–875
    [Google Scholar]
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