1887

Abstract

pWW53 is a 110 kbp catabolic plasmid which encodes the complete pathway for the utilization of toluene and the xylenes. The upper pathway operon is located between two homologous but distinct pathway operons, (), which are in direct repeat. These have each been cloned on large dIII restriction fragments HA (17.5 kbp) and HB (15.6 kbp), the restriction sites of which have been mapped. During growth of MT53 on benzoate, mutants which have lost the ability to grow on hydrocarbons such as -xylene (Mxy) but which retain the ability to grow on their carboxylic acid metabolites such as -toluate (Mtol) take over the culture before ultimately being displaced by plasmid-free strains which are Mxy Mtol. The plasmids in the Mxy Mtol mutants are formed by a large deletion between homologous regions of the two duplicate pathway operons. This causes the loss of the intervening operon and the formation of a hybrid () operon, starting with the genes originally on HA and terminating with the genes originally on HB.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-134-11-2965
1988-11-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/micro/134/11/mic-134-11-2965.html?itemId=/content/journal/micro/10.1099/00221287-134-11-2965&mimeType=html&fmt=ahah

References

  1. Bagdasarian M., Lurz R., RÜckert B., Franklin F.C.H., Bagdasarian M.M., Frey J., Timmis K.N. 1981; Specific purpose plasmid cloning vectors. II. Broad host range, high copy number RSFlOlO-derived vectors and a host: vector system for gene cloning.. Gene 16:237–247
    [Google Scholar]
  2. Bayley S.A., Duggleby C.J., Worsey M.J., Williams P.A., Hardy K.G., Broda P. 1977; Two modes of loss of the Tol function from Pseudomonas putida mt-2.. Molecular and General Genetics 154:203–204
    [Google Scholar]
  3. Chatfield L.K., Williams P.A. 1986; Naturally occurring TOL plasmids in Pseudomonas strains carry either two homologous or two nonhomologous catechol 2,3-oxygenase genes.. Journal of Bacteriology 168:878–885
    [Google Scholar]
  4. Doten R.C., Gregg L.A., Ornston L.N. 1987; Influence of the catBCE sequence on the phenotypic reversion of a pcaE mutation in Acinetobacter calcoaceticus.. Journal of Bacteriology 169:3175–3180
    [Google Scholar]
  5. Grinter N.J. 1983; A broad host range cloning vector transposable to various replicons.. Gene 21:133–143
    [Google Scholar]
  6. Holmes D.S., Quigley M. 1981; A rapid boiling method for preparation of bacterial plasmids.. Analytical Biochemistry 114:193–197
    [Google Scholar]
  7. Jeenes D.J., Williams P.A. 1982; Excision and integration of degradative pathway genes from TOL plasmid pWWO.. Journal of Bacteriology 150:188–194
    [Google Scholar]
  8. Keil H., Williams P.A. 1985; A new class of TOL plasmid deletion mutants in Pseudomonas putidaMT15 and their reversion by tandem gene amplification.. Journal of General Microbiology 131:1023–1033
    [Google Scholar]
  9. Keil H., Keil S., Pickup R.W., Williams P.A. 1985a; Evolutionary conservation of genes coding for meta pathway enzymes within TOL plasmids pWWOamd pWW53.. Journal of Bacteriology 164:887–895
    [Google Scholar]
  10. Keil H., Lebens M.R., Williams P.A. 1985b; TOL plasmid PWW15 contains two nonhomologous, independently regulated catechol 2-3-oxygenase genes.. Journal of Bacteriology 163:248–255
    [Google Scholar]
  11. Keil H., Keil S., Williams P.A. 1987; Molecular analysis of regulatory and structural xyl genes of the TOL plasmid pWW53-4.. Journal of General Microbiology 133:1149–1158
    [Google Scholar]
  12. Kunz D.A., Chapman P.J. 1981; Isolation and characterization of spontaneously occurring TOL plasmid mutants of Pseudomonas putida HS1.. Journal of Bacteriology 146:952–964
    [Google Scholar]
  13. Maniatis T., Fritsch E.F., Sambrook J. 1982 (editors) Molecular Cloning: a Laboratory Manual pp. 94–148 Cold Spring Harbor, New York:: Cold Spring Harbor Laboratory.;
    [Google Scholar]
  14. Meulien P., Downing R.G., Broda P. 1981; Excision of the 40 kb segment of the TOL plasmid from Pseudomonas putida mt-2 involves direct repeats.. Molecular and General Genetics 184:97–101
    [Google Scholar]
  15. Murray K., Williams P.A. 1974; Role of catechol and the methylcatechols as inducers of aromatic metabolism in Pseudomonas putida.. Journal of Bacteriology 117:1153–1157
    [Google Scholar]
  16. Murray N.E., Brammar W.J., Murray K. 1977; Lambdoid phages that simplify the recovery of in vitro recombinants.. Molecular and General Genetics 150:53–61
    [Google Scholar]
  17. Nakazawa T., Hayashi E., Yokota T., Ebina Y., Nakazawa A. 1978; Isolation of TOL and RP4 recombinants by integrative suppression.. Journal of Bacteriology 134:270–277
    [Google Scholar]
  18. Negoro S., Taniguchi T., Kanaoka M., Kimura H., Okada H. 1983; Plasmid determined enzymatic degradation of nylon oligomers.. Journal of Bacteriology 155:22–31
    [Google Scholar]
  19. Negoro S., Nakamura S., Okada H. 1984; DNA-DNA hybridization analysis of nylon oligomer-degradative plasmid pOAD2: identification of the DNA region analogous to the nylon oligomer degradation gene.. Journal of Bacteriology 158:419–424
    [Google Scholar]
  20. Ohno S. 1984; Birth of a unique enzyme from an alternative reading frame of the preexisted, internally repetitious coding sequence.. Proceedings of the National Academy of Sciences of the United States of America 81:2421–2425
    [Google Scholar]
  21. Pickup R.W. 1984 Physical and structural properties of TOL plasmids in Pseudomonas. PhD thesis University of Wales.:
    [Google Scholar]
  22. Sala-Trepat J.-M., Evans W.C. 1971; The metacleavage of catechol by Azotobacter species: 4-oxalocrotonate pathway.. European Journal of Biochemistry 20:400–413
    [Google Scholar]
  23. Shaw L.E., Williams P.A. 1988; Physical and functional mapping of two cointegrate plasmids derived from RP4 and TOL plasmid pDKl.. Journal of General Microbiology 134:2463–2474
    [Google Scholar]
  24. Sinclair M.I., Maxwell P.C., Lyon B.R., Holloway B.W. 1986; Chromosomal location of TOL plasmid DNA in Pseudomonas putida.. Journal of Bacteriology 168:1302–1308
    [Google Scholar]
  25. Southern E.M. 1975; Detection of specific sequences among DNA fragments separated by gel electrophoresis.. Journal of Molecular Biology 98:505–518
    [Google Scholar]
  26. Stephens G.M., Dalton H. 1987; The effect of lipophilic weak acids on the segregational stability of TOL plasmids in Pseudomonas putida.. Journal of General Microbiology 133:1891–1899
    [Google Scholar]
  27. Wheatcroft R., Williams P.A. 1981; Rapid methods for the study of both stable and unstable plasmids in Pseudomonas.. Journal of General Microbiology 124:433–437
    [Google Scholar]
  28. Williams P.A., Murray K. 1974; Metabolism of benzoate and the methylbenzoates by Pseudomonas putida [arvilla) mt-2: evidence for the existence of a TOL plasmid.. Journal of Bacteriology 120:416–423
    [Google Scholar]
  29. Williams P.A., Worsey M.J. 1976; Ubiquity of plasmids in coding for toluene and xylene metabolism in soil bacteria: evidence for the existence of new TOL plasmids.. Journal of Bacteriology 125:818–828
    [Google Scholar]
  30. Williams P.A., Taylor S.D., Gibb L.E. 1988; Loss of the toluene-xylene catabolic genes of TOL plasmid pWWO during growth of Pseudomonas putida on benzoate is due to a selective growth advantage of ‘cured’ segregants.. Journal of General Microbiology 134:2039–2048
    [Google Scholar]
  31. Worsey M.J., Williams P.A. 1975; Metabolism of toluene and xylenes by Pseudomonas putida (arvilla) mt-2: evidence for a new function of the TOL plasmid.. Journal of Bacteriology 124:7–13
    [Google Scholar]
  32. Yano K., Nishi T. 1980; pKJ1, a naturally occurring conjugative plasmid coding for toluene degradation and resistance to streptomycin and sulphonamides.. Journal of Bacteriology 143:552–560
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-134-11-2965
Loading
/content/journal/micro/10.1099/00221287-134-11-2965
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error