1887

Abstract

The activities of ATP: citrate lyase (ACL; EC 4.1.3.8), carnitine acetyltransferase (CAT; EC 2.3.1.7), NADP-dependent isocitrate dehydrogenase (ICDH; EC 1.1.1.42), isocitrate lyase (ICL; EC 4.1.3.1) and malic enzyme (malate dehydrogenase; EC 1.1.1.40) were measured in four oleaginous yeasts, D, and two strains of , grown either to accumulate lipid, or to utilize their own lipid reserves or an exogenous supply of lipid. During lipid utilization, activities of ACL and malic enzyme diminished to low levels; CAT and ICL increased considerably in activity and ICDH activity was slightly increased but catalase (EC 1.11.1.6) diminished in activity in both strains of In all cases, yeasts utilizing exogenous lipid showed greater changes in enzyme activities than cells utilizing their endogenous reserves. Electron micrographs of D showed proliferation of peroxisomes in starved cells utilizing their own lipid reserve though peroxisomes were more in evidence when the yeast had been grown on exogenous lipid. In , which shows only minimal utilization of its stored lipid and furthermore cannot grow on exogenous lipid, only the occasional peroxisome was seen when cells were starved of carbon.

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/content/journal/micro/10.1099/00221287-134-11-2907
1988-11-01
2021-05-13
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References

  1. Botham P.A., Ratledge C. 1979; A biochemical explanation for lipid accumulation in Candida 107 and other oleaginous micro-organisms.. Journal of General Microbiology 114:361–375
    [Google Scholar]
  2. Boulton C.A., Ratledge C. 1983a; Partial purification and some properties of ATP: citrate lyase from the oleaginous yeast Lipomyces cerevisiae.. Journal of General Microbiology 129:2863–2869
    [Google Scholar]
  3. Boulton C.A., Ratledge C. 1983b; Use of transition studies in continuous cultures of Lipomyces starkeyi, an oleaginous yeast, to investigate the physiology of lipid accumulation.. Journal of General Microbiology 129:2871–2876
    [Google Scholar]
  4. Boulton C.A., Ratledge C. 1984; The physiology of hydrocarbon-utilizing micro-organisms.. In Topics in Enzyme and Fermentation Biotechnology pp. 11–77 Wiseman A. Edited by Chichester: Ellis Horwood;
    [Google Scholar]
  5. Bradford M.M. 1976; A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.. Analytical Biochemistry 72:248–254
    [Google Scholar]
  6. Fukui S., Tanaka A. 1979a; Peroxisomes of alkane- and methanol-grown yeasts.. Journal of Applied Biochemistry 1:171–201
    [Google Scholar]
  7. Fukui S., Tanaka A. 1979b; Yeast peroxisomes.. Trends in Biochemical Sciences 4:246–249
    [Google Scholar]
  8. Fukui S., Tanaka A. 1981; Metabolism of alkanes by yeasts.. Advances in Biochemical Engineering 19:217–237
    [Google Scholar]
  9. Holdsworth J.E. 1987 Aspects of lipid metabolism in oleaginous yeasts. PhD thesis Hull University.:
    [Google Scholar]
  10. Holdsworth J.E., Ratledge C. 1986; Utilization of accumulated lipid by oleaginous yeasts.. XIth International Specialized Symposium on Yeasts, Lisbon p. 66:
    [Google Scholar]
  11. Holdsworth J.E., Ratledge C. 1987; Lipid turnover in oleaginous yeasts.. Journal of General Microbiology 134:339–346
    [Google Scholar]
  12. Hsu R.Y., Lardy H.A. 1969; Malic enzyme.. Methods in Enzymology 13:230–235
    [Google Scholar]
  13. Kohlaw G.B., Tan-Wilson A. 1977; Carnitine acetyltransferase: candidate for the transfer of acetyl groups through the mitochondrial membrane of yeast.. Journal of Bacteriology 129:1159–1161
    [Google Scholar]
  14. Kornberg A. 1955; Isocitrate dehydrogenase of yeast.. Methods in Enzymology 1:705–709
    [Google Scholar]
  15. Mcfadden B.A. 1969; Isocitrate lyase.. Methods in Enzymology 13:163–170
    [Google Scholar]
  16. Marshall B.J., Ratledge C., Norman E. 1973; Improved design for a simple and inexpensive multilace laboratory fermenter.. Laboratory Practice 22:491–492
    [Google Scholar]
  17. Naganuma T., Uzuka Y., Tanaka K., Iizuka H. 1987; Differences in enzyme activities of Lipomyces starkeyi between cells accumulating lipid and proliferating cells.. Journal of Basic Microbiology 27:35–42
    [Google Scholar]
  18. Ratledge C. 1986; The potential of micro-organisms for oil production - a review of recent publications.. In Proceedings of the World Conference on Emerging Technologies within the Fats and Oils Industry pp. 318–330 Baldwin A. R. Edited by Champaign, Illinois: American Oil Chemists’ Society;
    [Google Scholar]
  19. Ratledge C. 1987; Lipid biotechnology - a wonderland for the microbial physiologist.. Journal of the American Oil Chemists’ Society 64:1647–1656
    [Google Scholar]
  20. Ratledge C., Gilbert S.C. 1985; Carnitine acetyltransferase activity in oleaginous yeasts.. FEMS Microbiology Letters 27:273–275
    [Google Scholar]
  21. Srere P.A. 1962; Citrate cleavage enzyme.. Methods in Enzymology 5:641–644
    [Google Scholar]
  22. Teranishi Y., Kowamoto S., Tanaka A., Osumi M., Fukui S. 1974; Induction of catalase activity by hydrocarbons in Candida tropicalis.. Agricultural and Biological Chemistry 38:1221–1225
    [Google Scholar]
  23. Ueda M., Tanaka A., Fukui S. 1982; Peroxisomes and mitochondrial carnitine acetyltransfer- ases in alkane-grown yeast Candida tropicalis.. European Journal of Biochemistry 124:205–210
    [Google Scholar]
  24. Veenhuis M., Mateblowski M., Kunau W.H., Harder W. 1987; Proliferation of microbodies in Saccharomyces cerevisiae.. Yeast 3:77–84
    [Google Scholar]
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