RT Journal Article SR Electronic(1) A1 Morris, G. J. A1 Smith, D. A1 Coulson, G. E.YR 1988 T1 A Comparative Study of the Changes in the Morphology of Hyphae during Freezing and Viability upon Thawing for Twenty Species of Fungi JF Microbiology, VO 134 IS 11 SP 2897 OP 2906 DO https://doi.org/10.1099/00221287-134-11-2897 PB Microbiology Society, SN 1465-2080, AB The changes in morphology and viability of 20 species of fungi during freezing were examined in relation to cooling rate and the presence of glycerol. All of the hyphomycetes examined, the ascomycete Sordaria, the zygomycete Mucor, and the basidiomycete Schizophyllum, survived freezing and thawing in the absence of glycerol. Cryomicroscopy demonstrated that for these fungi the formation of intracellular ice at rapid rates of cooling was not lethal. Isolates from the oomycetes and some basidiomycetes required glycerol for survival. The morphological response of Phytophthora, Aschersonia and Volvariella differed from other genera, with shrinkage occurring at all rates of cooling. The conventionally employed cooling rate for the cryopreservation of fungi, 1 °C min−1, was found not to be optimal for all of the strains studied. Serpula recovered best after cooling at 0·5 °C min−1 followed by rapid thawing, and all the oomycetes examined gave highest recovery after cooling at rates between 5 and 10 °C min−1 followed by rapid thawing., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-134-11-2897