RT Journal Article SR Electronic(1) A1 Saulnier, Patrick A1 Hanquier, Jose A1 Jaoua, Samir A1 Reichenbach, Hans A1 Guespin-Michel, Janine F.YR 1988 T1 Utilization of IncP-1 Plasmids as Vectors for Transposon Mutagenesis in Myxobacteria JF Microbiology, VO 134 IS 11 SP 2889 OP 2895 DO https://doi.org/10.1099/00221287-134-11-2889 PB Microbiology Society, SN 1465-2080, AB No free plasmid has ever been found in the myxobacterium Myxococcus xanthus, but IncP-1 plasmids are able to integrate into the chromosome of this bacterium. The frequency of integration depends greatly upon the structure of the IncP-1 plasmid used. This property has been used to devise new delivery systems for transposon mutagenesis in this species. Plasmids with low integration efficiencies have proved to be efficient donors of Tn5, while plasmids with very high frequencies of integration could be used directly to generate mutations. These vectors have also proved efficient for Tn5 transfer into other species of myxobacteria, which have not so far been susceptible to genetic analysis., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-134-11-2889