1887

Abstract

No free plasmid has ever been found in the myxobacterium , but IncP-1 plasmids are able to integrate into the chromosome of this bacterium. The frequency of integration depends greatly upon the structure of the IncP-1 plasmid used. This property has been used to devise new delivery systems for transposon mutagenesis in this species. Plasmids with low integration efficiencies have proved to be efficient donors of Tn, while plasmids with very high frequencies of integration could be used directly to generate mutations. These vectors have also proved efficient for Tn transfer into other species of myxobacteria, which have not so far been susceptible to genetic analysis.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-134-11-2889
1988-11-01
2021-05-12
Loading full text...

Full text loading...

/deliver/fulltext/micro/134/11/mic-134-11-2889.html?itemId=/content/journal/micro/10.1099/00221287-134-11-2889&mimeType=html&fmt=ahah

References

  1. Appleyard R.K. 1954; Segregation of new lysogenic types during growth of a doubly lysogenic strain derived from Escherichia coli K12.. Genetics 39:440–444
    [Google Scholar]
  2. Breton A.M., Nicaud J.M., Younes G., Guespin-Michel J. 1984; Myxococcus xanthus, a Gram-negative non-pathogenic bacterium that secretes proteins into the extracellular growth medium, is a potential cloning host for excreted proteins production.. In Third European Congress on Biotechnology 3 pp. 441–446 Weinheim: Verlag Chemie;
    [Google Scholar]
  3. Breton A.M., Jaoua S., Guespin-Michel J. 1985; Transfer of plasmid RP4 to Myxococcus xanthus and evidence for its integration into the chromosome.. Journal of Bacteriology 161:523–528
    [Google Scholar]
  4. Campos J.M., Geiselsoder J., Zusman D. 1978; isolation of Bacteriophage Mx4, a generalized transducing phage for Myxococcus xanthus.. Journal of Molecular Biology 119:167–178
    [Google Scholar]
  5. Clewell D.B., Helinski D. 1969; supercoiled circular DNA protein complex in Escherichia coli'. induced conversion to an open circular DNA form.. Proceedings of the National Academy of Sciences of the United States of America 62:1139–1166
    [Google Scholar]
  6. Cohen S.N., Miller C.A. 1969; Multiple molecular species of circular R-factor DNA isolated from Escherichia coli.. Nature; London: 2241273–1276
    [Google Scholar]
  7. Eckhardt T. 1978; A rapid method for the identification of plasmid deoxyribonucleic acid in bacteria.. Plasmid 1:584–588
    [Google Scholar]
  8. Gerth K., Irschik H., Reichenbach H., Trowitzsch W. 1980; Myxothiazol, an antibiotic from Myxococcus fulvus. I. Cultivation, isolation, physio-chemical and biological properties.. Journal of Antibiotics 33:1474–1479
    [Google Scholar]
  9. Gerth K., Jansen R., Reifenstahl G., Hofle G., Irschik H., Kunze B., Reichenbach H., Thierbach G. 1983; The myxalamids, new antibiotics from Myxococcus xanthus (Myxobacterales).. Journal of Antibiotics 36:1150–1156
    [Google Scholar]
  10. Guespin-Michel J.F., Breton A., Nicaud J.M., Younes G. 1984; Etude genetique d’une bacterie a Gram negatif non pathogene, excretrice, Myxococcus xanthus.. In Genetique des Microorganismes Indus- triels pp. 337–341 Heslot H. Edited by Paris: Societe Francaise de Microbiologie;
    [Google Scholar]
  11. Hedges R.W., Matthew M. 1979; Acquisition by Escherichia coli of plasmid borne β-lactamases normally confined to Pseudomonas spp.. Plasmid 2:269–278
    [Google Scholar]
  12. Hodgkin J., Kaiser D. 1979; Genetics of gliding mobility in Myxococcus xanthus (Myxobacterales): genes controlling movement of single cells.. Molecular and General Genetics 171:167–176
    [Google Scholar]
  13. Jaoua S., Breton A.M., Younes G., Guespin-Michel J.F. 1986; Structural instability and stabilization of IncP-1 plasmids integrated into the chromosome of Myxococcus xanthus.. Journal of Biotechnology 4:313–323
    [Google Scholar]
  14. Jaoua S., Guespin-Michel J.F., Breton A. 1987; Mode of insertion of the broad-host-range plasmid RP4 and its derivates into the chromosome of Myxococcus xanthus.. Plasmid 18:111–119
    [Google Scholar]
  15. Kaiser D., Dworkin M. 1975; Gene transfer to a myxobacterium by Escherichia coli phage P1.. Science 187:563–564
    [Google Scholar]
  16. Kaiser D., Manoil C., Dworkin M. 1979; Myxobacteria: cell interactions, genetics and development.. Annual Review of Microbiology 33:595–639
    [Google Scholar]
  17. Kopecko D.J., Brevet J., Cohen S.N. 1976; Involvement of multiple translocating DNA segments and recombinational hot-spots in the structural evolution of bacterial plasmids.. Journal of Molecular Biology 108:333–360
    [Google Scholar]
  18. Kuner J.M., Kaiser D. 1981; Introduction of transposon Tn5 into Myxococcus xanthus for analysis of developmental and other non-selectable mutants.. Proceedings of the National Academy of Sciences of the United States of America 78:425–429
    [Google Scholar]
  19. Mazodier P., Cossart P., Giraud F., Gaser F. 1985; Completion of the nucleotide sequence of the central region of Tn5 confirms the presence of three resistance genes.. Nucleic Acids Research 13:195–205
    [Google Scholar]
  20. Miller J. 1972 Experiments in Molecular Genetics. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  21. Nicaud J.M., Breton A.M., Younes G., Guespin-Michel J.F. 1984; Mutants of Myxococcus xanthus impaired in protein secretion: an approach to study a secretory mechanism.. Applied Microbiology and Biotechnology 20:344–350
    [Google Scholar]
  22. Reichenbach H., Gerth K., Irschik H., Kunze B., Hofle G. 1988; Myxobacteria, a source for new antibiotics.. In Biotechnology of Antibiotics, Steroids and Alkaloids of Medical Importance. Vlahov R. Edited by Weinheim: VCH Verlagsgesellschaft; (in the Press)
    [Google Scholar]
  23. Rella M. 1984 Transposon insertion mutagenesis of the Pseudomonas aeruginosa genome. Thesis Swiss Federal Institute of Technology; Zurich:
    [Google Scholar]
  24. Rosenberg E., Porter J.M., NATHAN P.N., Manor A., Varon M. 1984; Antibiotic TA: an adherent antibiotic.. Biotechnology 2:796–799
    [Google Scholar]
  25. Shimkets L.J., Gill R.E., Kaiser D. 1983; Developmental cell interactions in Myxococcus xanthus and the spoC locus.. Proceedings of the National Academy of Sciences of the United States of America 80:1406–1410
    [Google Scholar]
  26. Southerne M. 1975; Detection of specific sequences among DNA fragments separated by gel electrophoresis.. Journal of Molecular Biology 98:503–517
    [Google Scholar]
  27. Zusman D.R., Krotoski D.M., Cumsky M. 1978; Chromosome replication in Myxococcus xanthus.. Journal of Bacteriology 133:122–129
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-134-11-2889
Loading
/content/journal/micro/10.1099/00221287-134-11-2889
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error