1887

Abstract

Summary: The effects of - and -methionine on production of the gaseous secondary metabolite chloromethane (CHC1) by the white-rot fungus were investigated. Although -methionine stimulated CHC1 biosynthesis, the -isomer either failed to affect or depressed production depending on the concentration. Nevertheless, very high levels of incorporation of label (~80%) from -[-H]methionine into CHC1 could be achieved demonstrating that the amino acid is a highly effective precursor of CHC1. Experiments using labelled -methionine showed substantial but lower incorporation. Patterns of incorporation of label from both isomers into methyl benzoate, another important volatile fungal metabolite, were very similar to those observed with CHC1. This behaviour is only explicable in terms of either () the utilization of the same enzyme system requiring an identical methyl donor for methylation of both chloride and benzoate ions or () the presence of a transmethylation system. The incorporation of label from -[-H]methionine into methyl 2-furoate was similar to that of methyl benzoate implying that the same methylating system was used for esterification of both 2-furoic and benzoic acids. However, the incorporation pattern for methyl salicylate was different and suggests that methyl salicylate is probably formed by -hydroxylation of methyl benzoate.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-134-10-2831
1988-10-01
2021-07-24
Loading full text...

Full text loading...

/deliver/fulltext/micro/134/10/mic-134-10-2831.html?itemId=/content/journal/micro/10.1099/00221287-134-10-2831&mimeType=html&fmt=ahah

References

  1. Cowan M. I., Glen A. T., Hutchinson S. A., Maccartney M. E., Mackintosh J. M., Moss A. M. 1973; Production of volatile metabolites by species of Fomes. . Transactions of the British Mycological Society 60:347–356
    [Google Scholar]
  2. Dirar H. A., Harper D. B., Collins M. A. 1985; Biochemical and microbiological studies on kawal a meat substitute derived by fermentation of Cassia obtusifolia leaves. Journal of the Science of Food and Agriculture 36:881–892
    [Google Scholar]
  3. Harper D. B. 1985; Halomethane from halide ion - a highly efficient fungal conversion of environmental significance. Nature, London 315:55–57
    [Google Scholar]
  4. Harper D. B., Kennedy J. T. 1986; Effect of growth conditions on halomethane production by Phellinus species: biological and environmental implications. Journal of General Microbiology 132:1231–1246
    [Google Scholar]
  5. Harper D. B., Kennedy J. T., Hamilton J. T. G. 1988; Chloromethane synthesis in poroid fungi. Phytochemistry in the Press
    [Google Scholar]
  6. Neidleman S. L., Geigert J. 1986 Biohalogena- tion: Principles, Basic Roles and Applications Chichester: Ellis Horwood;
    [Google Scholar]
  7. Theiler R., Cook J. C., Hager L. P., Siuda J. F. 1978; Halohydrocarbon synthesis by bromoperoxidase. Science 202:1094–1096
    [Google Scholar]
  8. White R. H. 1982; Biosynthesis of methyl chloride in the fungus, Phellinus pomaceus. . Archives of Microbiology 132:100–102
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-134-10-2831
Loading
/content/journal/micro/10.1099/00221287-134-10-2831
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error