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Abstract

The genes encoding the -subunits of ATP-synthases (ATPases) from DSM 2151, DSM 2039 and were cloned. The nucleotide sequences were determined completely for the genes of the first two organisms and to a major part for that of ‘’. The predicted amino acid sequences were compared with previously published amino acid sequences of -subunits. Two characteristic insertions were found in genes from organisms belonging to the so-called bacteroides-cytophaga-flavo-bacterium group. The remaining structure shows a high degree of sequence similarity within this group. These data support the conclusions drawn from comparative 16S rRNA sequence analyses that organisms in this phenotypically heterogeneous group are phylogenetically related. A phylogenetic tree was constructed based on a distance matrix of optimally aligned amino acid sequences of -subunits of ATPases of various eubacteria, chloroplasts and mitochondria. It is in good agreement with a tree derived from 16S rRNA sequence analyses.

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/content/journal/micro/10.1099/00221287-134-10-2815
1988-10-01
2025-03-24
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References

  1. Amann R., Sostak P., Ludwig W., Schleifer K. H. 1988; Cloning and sequencing of genes encoding the beta subunits of the ATP-synthases of Enterobacter aerogenes and Flavobacterium ferrugineum . FEMS Microbiology Letters 50:101–106
    [Google Scholar]
  2. Bolivar F., Rodriguez R., Greene P. J., Betlach M., Heynecker H. L., Bayer H. W., Crosa J., Falkow S. 1977; Construction and characterization of new cloning vehicles. II. A multipurpose cloning system. Gene 2:95–113
    [Google Scholar]
  3. Chen E. Y., Seeburg P. H. 1985; Supercoiled sequencing: a fast and simple method for sequencing plasmid DNA. DNA 4:165–170
    [Google Scholar]
  4. Cozens A. L., Walker J. E. 1987; The organization and sequence of the genes for ATP-synthase subunits in the cyanobacterium Synechococcus 6301. Journal of Molecular Biology 194:359–383
    [Google Scholar]
  5. Curtis S. E. 1987; Genes encoding Beta and Epsilon subunits of the proton-translocating ATPase from Anabaena sp. strain PCC 7120. Journal of Bacteriology 169:80–86
    [Google Scholar]
  6. Falk G., Hampe A., Walker J. E. 1985; Nucleotide sequence of the Rhodospirillum rubrum atp operon. Biochemical Journal 228:391–407
    [Google Scholar]
  7. Fitch W. M., Margoliash E. 1967; Construction of phylogenetic trees. Science 155:279–284
    [Google Scholar]
  8. Futai M., Kanazawa H. 1983; Structure and function of proton-translocating adenosine triphosphatase (F0F1): biochemical and molecular biological approach. Microbiological Reviews 47:285–312
    [Google Scholar]
  9. Hennecke H., Günther I., Binder F. 1982; A novel cloning vector for the direct selection of recombinant DNA in Escherichia coli . Gene 19:231–234
    [Google Scholar]
  10. Kagawa Y., Ishizuka M., Saishu T., Nakao S. 1986; Stable structure of thermophilic proton ATPase beta-subunit. Journal of Biochemistry 100:923–934
    [Google Scholar]
  11. Marmur J. 1961; A procedure for the isolation of DNA from microorganisms. Journal of Molecular Biology 3:208–218
    [Google Scholar]
  12. Meyer S. A., Schleifer K. H. 1978; Deoxyribonucleic acid reassociation in the classification of coagulase-positive staphylococci. Archives of Microbiology 117:183–188
    [Google Scholar]
  13. Paster B. J., Ludwig W., Weisburg W. G., Stackebrandt E., Hespell R. B., Hahn C. M., Reichenbach H., Stetter K. O., Woese C. R. 1985; A phylogenetic grouping of the bacteroides, cytophagas and certain flavobacteria. Systematic and Applied Microbiology 6:34–42
    [Google Scholar]
  14. Runswick M. J., Walker J. E. 1983; The amino acid sequence of the beta subunit of ATP-synthase from bovine heart mitochondria. Journal of Biological Chemistry 258:9307–9313
    [Google Scholar]
  15. Sanger F., Nickler S., Coulson A. R. 1977; DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences of the United States of America 74:5463–5467
    [Google Scholar]
  16. Saraste M., Gay N. J., Eberle A., Runswick M. J., Walker J. E. 1981; The atp operon: nucleotide sequence of the genes for the γ,β and εsubunits of Escherichia coli ATP-synthase. Nucleic Acids Research 9:5287–5296
    [Google Scholar]
  17. Shinozaki K., Yanada C., Takahata N., Sugiura M. 1983; Overlap and cotranscription of the genes for the beta and epsilon subunits of tobacco chloroplast ATPase. Gene 24:147–155
    [Google Scholar]
  18. Southern E. M. 1975; Detection of specific sequences among DNA fragments separated by gel electrophoresis. Journal of Molecular Biology 98:503–517
    [Google Scholar]
  19. Tybulewicz V. L. J., Falk G., Walker J. E. 1984; Rhodopseudomonas blastica atp operon: nucleotide sequence and transcription. Journal of Molecular Biology 178:185–214
    [Google Scholar]
  20. Walker J. E., Saraste M., Gay N. J. 1984; The unc operon: nucleotide sequence, regulation and structure of ATP-synthase. Biochimica et biophysica acta 768:164–200
    [Google Scholar]
  21. Weisburg W. G., Oyaizu Y., Oyaizu H., Woese C. R. 1985; Natural relationship between Bacteroides and Flavobacteria . Journal of Bacteriology 164:230–236
    [Google Scholar]
  22. Woese C. R. 1987; Bacterial evolution. Microbiological Reviews 51:221–271
    [Google Scholar]
  23. Yanisch-Perron C., Vieira J., Messing J. 1985; Improved Ml3 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectors. Gene 33:103–119
    [Google Scholar]
  24. Zilberstein D., Shirvan M. H., Barile M. F., Rottem S. 1986; The β-subunit of the FjF0- ATPase is conserved in mycoplasmas. Journal of Biochemistry 261:7109–7111
    [Google Scholar]
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