1887

Abstract

A number of strains of biovar were examined for variation in Sym plasmid size and number, using an probe. Size variation was great and, in a large proportion of strains, multiple Sym plasmids were observed. A probe containing a repeated sequence (RtRS) incorporating the promoter, reported to be specifically found on the Sym plasmid of this biovar, was used to determine whether Sym plasmids were of origin. This probe did not bind to all Sym plasmids identified by the probe and some isolates were suspected of being inter-biovar hybrids. The RtRS probe was also used to test certain isolates for DNA homology to the promoter region. Many strains possessed commonly occurring co-migrating fragments that hybridized to this probe. Beyond this basic relatedness, greater variation in fragment number and size existed, allowing differentiation of seemingly closely related strains. Strains carrying greater amounts of symbiotic DNA in the form of multiple Sym plasmids or a greater number of hybridizing fragments were less effective in fixing nitrogen.

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1988-10-01
2021-10-27
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References

  1. Better M., Lewis B., Corbin D., Ditta G., Helinski D. R. 1983; Structural relationships among Rhizobium meliloti symbiotic promoters. Cell 35:479–485
    [Google Scholar]
  2. Brewin N. J., Dejong T. M., Phillips D. A., Johnston A. W. B. 1980; Co-transfer of determinants for hydrogenase activity and nodulation ability in Rhizobium leguminosarum . Nature; London: 28877–79
    [Google Scholar]
  3. Christensen A. H., Schubert K. R. 1983; Identification of a Rhizobium trifolii plasmid coding for nitrogen fixation and nodulation genes and its interaction with pJB5JI, a Rhizobium leguminosarum plasmid. Journal of Bacteriology 156:592–599
    [Google Scholar]
  4. Djordevic M. A., Zurkowski W., Rolfe B. G. 1982; Plasmids and stability of symbiotic properties in Rhizobium trifolii . Journal of Bacteriology 151:560–568
    [Google Scholar]
  5. Downie J. A., Ma Q. S., Knight C. D., Hom-Brecher G., Johnston A. W. B. 1983; Cloning of the symbiotic region of Rhizobium leguminosarum: the nodulation genes are between the nitrogenase genes and a nifA-like gene. EMBO Journal 2:947–952
    [Google Scholar]
  6. Eckhardt T. 1978; A rapid method for the identification of plasmid deoxyribonucleic acid in bacteria. Plasmid 1:584–588
    [Google Scholar]
  7. Gotz R., Evans I. J., Downie J. A., Johnston A. W. B. 1985; Identification of the host range DNA which allows Rhizobium leguminosarum strain TOM to nodulate cv. Afghanistan peas. Molecular and General Genetics 201:296–300
    [Google Scholar]
  8. Hirsch P. R., Van Montagu M., Johnston A. W. B., Brewin N. J., Schell J. 1980; Physical identification of bacteriocinogenic, nodulation and other plasmids in strains of Rhizobium leguminosarum . Journal of General Microbiology 120:403–412
    [Google Scholar]
  9. Hombrecher G., Brewin N. J., Johnston A. W. B. 1981; Linkage of genes for nitrogenase and nodulation ability on plasmids in Rhizobium leguminosarum and R. phaseoli . Molecular and General Genetics 182:133–136
    [Google Scholar]
  10. Hooykaas P. J. J., Van Brussel A. A. N., Dendulk-Ras H., Vanslogteren G. M. S., Schilperoort R. A. 1981; Sym plasmid of Rhizobium trifolii expressed in different rhizobial species and Agrobacterium tumefaciens . Nature; London: 291351–353
    [Google Scholar]
  11. Jensen H. L. 1942; Nitrogen fixation in leguminous plants I. General characters of root nodule bacteria isolated from species of Medicago and Trifolium in Australia. Proceedings of the Linnean Society of New South Wales 66:98–108
    [Google Scholar]
  12. Johnston A. W. B., Beynon J. L., Buchanan-Wollaston A. V., Setchell S. M., Hlrsch P. R., Beringer J. E. 1978; High frequency transfer of nodulating ability between strains and species of Rhizobium . Nature; London: 276634–636
    [Google Scholar]
  13. Jordan D. C. 1984; Family III. Rhizobiaceae. In Bergey’s Manual of Systematic Bacteriology 365 pp. 234–256 Krieg N. R. Edited by Baltimore & London: Williams & Wilkins;
    [Google Scholar]
  14. Krol A. J. M., Hontelez J. G. J., Vankammen A. 1982; Only one of the large plasmids in Rhizobium leguminosarum strain PRE is strongly expressed in the endosymbiotic state. Journal of General Microbiology 128:1839–1847
    [Google Scholar]
  15. Van Larebeke N., Engler G., Holsters M., Van De Nelsacker S., Zaenen I., Schilperoort R. A., Schell J. 1974; A large plasmid in Agrobacterium tumefaciens essential for crown-gall inducing ability. Nature; London: 252169–170
    [Google Scholar]
  16. Norrander J., Kempe T., Messing J. 1983; Construction of improved Ml3 vectors using oligonucleotide-directed mutagenesis. Gene 26:101–106
    [Google Scholar]
  17. Nuti M. P., Lepidi A. A., Prakash R. K., Schilperoort R. A., Cannon F. C. 1979; Evidence of nitrogen fixation (nif) genes on indigenous Rhizobium plasmids. Nature; London: 282533–535
    [Google Scholar]
  18. Ronson C. W., Scott D. B. 1983; Identification, broad host range mobilization and mutagenesis of a Rhizobium trifolii Sym:: R68.45 cointegrate plasmid. In Molecular Genetics of Bacteria-Plant Interaction pp. 177–187 Puhler A. Edited by Berlin & Heidelberg: Springer;
    [Google Scholar]
  19. Ruvkun G. B., Asubel F. M. 1980; Interspecies homology of nitrogenase genes. Proceedings of the National Academy of Sciences of the United States of America 77:191–195
    [Google Scholar]
  20. Schofield P. R., Djordevic M. A., Rolpe B. G., Shine J., Watson J. M. 1983; A molecular linkage map of nitrogenase and nodulation genes in Rhizobium trifolii . Molecular and General Genetics 192:459–465
    [Google Scholar]
  21. Schofield P. R., Gibson A. H., Dudman W. F., Watson J. M. 1987; Evidence for genetic exchange and recombination of Rhizobium symbiotic plasmids in a soil population. Applied and Environmental Microbiology 53:2942–2947
    [Google Scholar]
  22. Scott K. F., Rolfe B. G., Shine J. 1983; Biological nitrogen fixation: primary structure of the Rhizobium trifolii iron protein gene. DNA 2:149–155
    [Google Scholar]
  23. Thurman N. P., Lewis D. M., Jones D. G. 1985; The relationship of plasmid number to growth, acid tolerance and symbiotic efficiency in isolates of Rhizobium trifolii . Journal of Applied Bacteriology 58:1–6
    [Google Scholar]
  24. Wang C. L., Hirsch P. R., Beringer J. E. 1986; Host plant effects on hybrids of Rhizobium leguminosarum biovars viceae and trifolii . Journal of General Microbiology 132:2063–2070
    [Google Scholar]
  25. Watson J. M., Schofield P. R. 1985; Species- specific, symbiotic plasmid located repeated DNA sequences in Rhizobium trifolii . Molecular and General Genetics 199:279–289
    [Google Scholar]
  26. Zurkowski W. 1982; Molecular mechanism for loss of nodulation properties of Rhizobium trifolii . Journal of Bacteriology 150:999–1007
    [Google Scholar]
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