Nonbactericidal Antibodies against : Evaluation of Their Blocking Effect on Bactericidal Antibodies Directed against Outer Membrane Antigens Free

Abstract

Nonbactericidal monoclonal antibodies (MAbs) directed against gonococcal surface antigens were examined for their effect on complement-mediated bactericidal killing by other MAbs and normal human serum. One MAb, SM73, directed against the H.8 antigen activated complement only moderately well and had little influence on bactericidal antibodies. Two antibodies directed against an epitope on protein III had very different effects. Antibody SM51 activated complement poorly and had no effect on bactericidal killing, whereas antibody SM50, although itself nonbactericidal, activated complement and blocked the bactericidal effect of other antibodies. The extent of the blocking ability of MAb SM50 was studied using MAbs of different specificities as well as polyclonal antisera raised aginst gonococcal surface antigens. Antibody SM50 blocked IgG MAbs of all specificities, but several MAbs of the IgM class retained their bactericidal effect. Each of these IgM MAbs reacted with lipopolysaccharide, but with different epitopes.

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1988-10-01
2024-03-28
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References

  1. Britigan B. E., Cohen M. S., Sparling P. F. 1986; Gonococcal infection: a model of molecular pathogenesis. New England Journal of Medicine 312:1683–1694
    [Google Scholar]
  2. Diaz J.-L., Heckels J. E. 1982; Antigenic variation of outer membrane protein II in colonial variants of Neisseria gonorrhoeae P9. Journal of General Microbiology 128:585–591
    [Google Scholar]
  3. Fletcher J. N., Zak K., Virji M., Heckels J. E. 1987; . Monoclonal antibodies to gonococcal outer membrane protein I: location of a conserved epitope on protein I.B. Journal of General Microbiology 132:1611–1620
    [Google Scholar]
  4. Heckels J. E. 1977; The surface properties of Neisseria gonorrhoeae: isolation of the major components of the outer membrane. Journal of General Microbiology 99:333–341
    [Google Scholar]
  5. Heckels J. E., Virji M. 1986; Antigenic variation of gonococcal surface proteins: effect on virulence. In Protein-Carbohydrate Interactions in Biological Systems pp. 89–93 Lark D. L. Edited by London: Academic Press;
    [Google Scholar]
  6. Joiner K. A., Warren K. A., Brown E. J., Swanson J., Frank M. M. 1983; Studies on the mechanism of bacterial resistance to complement- mediated killing. IV. C5b-9 forms higher molecular weight complexes with bacterial outer membrane constituents on serum resistant but not on serum sensitive Neisseria gonorrhoeae . Journal of Immunology 131:1443–1451
    [Google Scholar]
  7. Joiner K. A., Warren K. A., Tam M., Frank M. M. 1985a; Monoclonal antibodies directed against gonococcal protein I vary in bactericidal activity. Journal of Immunology 134:3411–3419
    [Google Scholar]
  8. Joiner K. A., Scales R., Warren K. A., Frank M. M., Rice P. A. 1985b; Mechanism of action of blocking Immunoglobulin G for Neisseria gonorrhoeae . Journal of Clinical Investigation 76:1765–1772
    [Google Scholar]
  9. Kaminski M. S., Kitamura K., Maloney D. G., Campbell M. J., Levy R. 1985; Importance of antibody isotype in monoclonal anti-idiotype therapy of a murine B cell lymphoma. A study of hybridoma class switch variants. Journal of Immunology 136:1123–1130
    [Google Scholar]
  10. Lytton E. J., Blake M. S. 1986; Isolation and partial characterisation of the reduction-modifiable protein of Neisseria gonorrhoeae . Journal of Experimental Medicine 164:1749–1759
    [Google Scholar]
  11. Mcshan W. M., Williams R. P., Hull R. A. 1988; . A recombinant molecule from a disseminating strain of Neisseria gonorrhoeae that confers serum bactericidal resistance. Infection and Immunity 55:3017–3022
    [Google Scholar]
  12. Neuberger M. S., Rajewsky K. 1981; Activation of mouse complement by monoclonal mouse antibodies. European Journal of Immunology 11:1012–1016
    [Google Scholar]
  13. Rice P. A., Goldenberg D. A. 1981; Clinical manifestations of disseminated infection caused by Neisseria gonorrhoeae and differences in bactericidal reactivity of infecting strains. Annals of Internal Medicine 95:175–178
    [Google Scholar]
  14. Rice P. A., Vayo H. E., Tam M. R., Blake M. S. 1986; Immunoglobulin G antibodies directed against protein III block killing of serum resistant Neisseria gonorrhoeae by immune serum. Journal of Experimental Medicine 164:1735–1748
    [Google Scholar]
  15. Schoolnik G. K., Buchanan T. M., Holmes K. K. 1976; Gonococci causing disseminated infection are resistant to the bactericidal action of normal human serum. Journal of Clinical Investigation 58:1163–1173
    [Google Scholar]
  16. Virji M., Heckels J. E. 1985; Role of anti-pilus antibodies in host defence against gonococcal infection studied with monoclonal anti-pilus antibodies. Infection & Immunity 49:621–628
    [Google Scholar]
  17. Virji M., Heckels J. E. 1986; The effect of protein II and pili on the interaction of Neisseria gonorrhoeae with human polymorphonuclear leucocytes. Journal of General Microbiology 132:503–512
    [Google Scholar]
  18. Virji M., Heckels J. E., Watt P. J. 1983; Monoclonal antibodies to gonococcal pili: studies on antigenic determinants on pili from variants of strain P9. Journal of General Microbiology 129:1965–1973
    [Google Scholar]
  19. Virji M., Zak K., Heckels J. E. 1985; Use of monoclonal antibodies to detect common protein antigens present in outer membranes of Neisseria species. In The Pathogenic Neisseriae pp. 276–280 Schoolnik G. K. Edited by Washington, DC: American Society for Microbiology;
    [Google Scholar]
  20. Virji M., Zak K., Heckels J. E. 1986; Monoclonal antibodies to gonococcal outer membrane protein IB: use in the investigation of the potential protective effect of antibodies directed against conserved and type-specific epitopes. Journal of General Microbiology 132:1621–1629
    [Google Scholar]
  21. Virji M., Zak K., Heckels J. E. 1987a; Outer membrane protein III of Neisseria gonorrhoeae: variations in biological properties of antibodies directed against different epitopes. Journal of General Microbiology 133:3393–3401
    [Google Scholar]
  22. Virji M., Fletcher J. N., Zak K., Heckels J. E. 1987b; The potential protective effect of monoclonal antibodies to gonococcal outer membrane protein IA. Journal of General Microbiology 133:2639–2646
    [Google Scholar]
  23. Zak K., Diaz J.-L., Jackson D., Heckels J. E. 1984; Antigenic variation during infection with Neisseria gonorrhoeae: detection of antibodies to surface proteins in sera of patients with gonorrheae. Journal of Infectious Diseases 149:166–174
    [Google Scholar]
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