1887

Abstract

The gas vesicles of the cyanobacteria and sp. contain, in addition to the principal 7400 gas vesicle protein GVPa, a second, 22000 protein component, GVPc. We show here that GVPc can be removed from the gas vesicles, without their collapsing, by rinsing in solutions of sodium dodecyl sulphate. It is concluded that GVPc is located on the outer surface of the hollow shell formed by GVPa. Removing GVPc causes a marked decrease in the critical collapse pressure of the gas vesicles. It is suggested that the protein provides structural support and reduces pressures generated by surface tension. By measurement of the S-labelling of gas vesicles we demonstrate that GVPc, which contains two methionine residues per molecule, accounts for 9% of the total gas vesicle protein, confirming the value suggested by amino acid analysis. P-labelling indicates that the phosphate content of gas vesicle protein is negligible.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-134-10-2647
1988-10-01
2022-01-24
Loading full text...

Full text loading...

/deliver/fulltext/micro/134/10/mic-134-10-2647.html?itemId=/content/journal/micro/10.1099/00221287-134-10-2647&mimeType=html&fmt=ahah

References

  1. Armstrong R.E., Hayes P.K., Walsby A.E. 1983; Gas vacuole formation in hormogonia of Nostoc muscorum.. Journal of General Microbiology 128:263–270
    [Google Scholar]
  2. Blaurock A.E., Walsby A.E. 1976; Crystalline structure of the gas vesicle wall from Anabaena flos- aquae.. Journal of Molecular Biology 105:183–199
    [Google Scholar]
  3. Buckland B., Walsby A.E. 1972; A study of the strength and stability of gas vesicles isolated from a blue-green alga.. Archiv fur Mikrobiologie 79:327–337
    [Google Scholar]
  4. Damerval T., Houmard J., Guglielmi G., Csiszar K., Tandeau de marsac N. 1987; A developmentally regulated gvpABC operon is involved in the formation of gas vesicles in the cyanobacterium Calothrix 7601.. Gene 54:83–92
    [Google Scholar]
  5. Dassarma S., Damerval T., Jones J.G., Tandeau de marsac N. 1987; A plasmid-encoded gas vesicle protein gene in a halophilic archaebacterium.. Molecular Microbiology 1:365–370
    [Google Scholar]
  6. Dinsdale M.T., Walsby A.E. 1972; The interrelations of cell turgor pressure, gas-vacuola- tion, and buoyancy in a blue-green alga.. Journal of Experimental Botany 23:561–570
    [Google Scholar]
  7. Hayes P.K., Walsby A.E., Walker J.E. 1986; Complete amino acid sequence of cyanobacterial gas-vesicle protein indicates a 70-residue molecule that corresponds in size to the crystallographic unit cell.. Biochemical Journal 236:31–36
    [Google Scholar]
  8. Hayes P.K., Lazarus C.M., Bees A., Walker J.E., Walsby A.E. 1988; The protein encoded by gvpC is a minor component of gas vesicles isolated from the cyanobacteria Anabeana flos-aquae and Microcystis sp.. Molecular Microbiology (in the press)
    [Google Scholar]
  9. Konopka A.E., Staley J.T., Lara J.C. 1975; Gas vesicle assembly in Microcyclus aquaticus.. Journal of Bacteriology 122:1301–1309
    [Google Scholar]
  10. Laemmli U.K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4.. Nature; London: 277680–685
    [Google Scholar]
  11. Oliver R.L., Walsby A.E. 1984; Direct evidence for the role of light-mediated gas vesicle collapse in the buoyancy regulation of Anabaena flos-aquae (cyanobacteria).. Limnology and Oceanography 29:879–886
    [Google Scholar]
  12. Olson F.C.W. 1950; Quantitative estimates of filamentous algae.. Transactions of the American Microscopical Society 59:272–279
    [Google Scholar]
  13. Rippka R., Deruelles J., Waterbury J.B., Herdman M., Stanier R.Y. 1979; Generic assignments, strain histories and properties of pure cultures of cyanobacteria.. Journal of General Microbiology 111:1–61
    [Google Scholar]
  14. Tandeau de marsac N., Mazel D., Bryant D.A., Houmard J. 1985; Molecular cloning and nucleotide sequence of a developmentally regulated gene from the cyanobacterium Calothrix PCC 7601: a gas vesicle protein gene.. Nucleic Acids Research 13:7223–7236
    [Google Scholar]
  15. Walker J.E., Walsby A.E. 1983; Molecular weight of gas-vesicle protein from the planktonic cyanobacterium Anabaena flos-aquae and implications for structure of the vesicle.. Biochemical Journal 209:809–815
    [Google Scholar]
  16. Walker J.E., Hayes P.K., Walsby A.E. 1984; Homology of gas vesicle proteins in cyanobacteria and halobacteria.. Journal of General Microbiology 130:2709–2715
    [Google Scholar]
  17. Walsby A.E. 1971; The pressure relationships of gas vacuoles.. Proceedings of the Royal Society of London B 178:301–326
    [Google Scholar]
  18. Walsby A.E. 1973; A portable apparatus for measuring relative gas vacuolation, the strength of gas vacuoles, and turgor pressure in planktonic blue- green algae and bacteria.. Limnology and Oceanography 18:653–658
    [Google Scholar]
  19. Walsby A.E. 1974; The isolation of gas vesicles from blue-green algae.. Methods in Enzymology 31A678–686 Fleischer S., Packer L. Edited by New York: Academic Press;
    [Google Scholar]
  20. Walsby A.E. 1982; The elastic compressibility of gas vesicles.. Proceedings of the Royal Society of London B126:355–368
    [Google Scholar]
  21. Walsby A.E. 1987; Mechanisms of buoyancy regulation by planktonic cyanobacteria with gas vesicles.. In The Cyanobacteria pp. 377–392 Fay P., Van Baalen C. Edited by Amsterdam: Elsevier Science Publishers;
    [Google Scholar]
  22. Walsby A.E., Armstrong R.E. 1979; Average thickness of the gas vesicle wall.. Journal of Molecular Biology 129:279–285
    [Google Scholar]
  23. Walsby A.E., Bleything A. 1988; The dimensions of cyanobacterial gas vesicles in relation to their efficiency in providing buoyancy and withstanding pressure.. Journal of General Microbiology 134:2635–2645
    [Google Scholar]
  24. Walsby A.E., Buckland B. 1969; Isolation and purification of intact gas vesicles from a blue-green alga.. Nature; London: 224716–717
    [Google Scholar]
  25. Weathers P.J., Jost M., Lamport D.T.A. 1977; The gas vacuole membrane of Microcystis aeruginosa.. Archives of Biochemistry and Biophysics 178:226–244
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-134-10-2647
Loading
/content/journal/micro/10.1099/00221287-134-10-2647
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error