1887

Abstract

The cyclomaltodextrin glucanotransferase (CGTase, EC 2.4.1.19) gene from the alkalophilic sp. strain no. 38-2 was cloned in using pBR322. A plasmid, pCS8, was isolated from a transformant producing CGTase and the cloned CGTase gene was found to be in a 5·3 kb DNA fragment. The nucleotide sequence of a 2·5 kb segment encoding the CGTase was determined. This segment showed an open reading frame which would encode a polypeptide of 712 amino acids. The pCS8 CGTase had the same enzymic properties as those of the extracellular CGTase produced by the alkalophilic sp. strain no. 38-2. The nucleotide and amino acid sequences of this CGTase gene and gene product, respectively, have strong homology with those of the CGTase.

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1988-01-01
2024-11-03
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References

  1. Béguin P., Cornet P., Aubert J.-P. 1985; Sequence of a cellulase gene of the thermophilic bacterium Clostridium thermocellum. Journal of Bacteriology 162:102–105
    [Google Scholar]
  2. Bender H. 1977; Cyclodextrin Glucanotransferase von Klebsiella pneumoniaeM501. Archives of Microbiology 111:271–282
    [Google Scholar]
  3. Bolivar E., Rodriguez R.L., Greene P.J., Betlach M.C., Heynecker H.L., Boyer H.W. 1977; Construction and characterization of new cloning vehicles. II. A multi-purpose cloning system. Gene 2:95–113
    [Google Scholar]
  4. Davis R.W., Botstein D., Roth J.R. 1980; Advanced bacterial genetics. In A Manual for Genetic Engineering pp. 120–121 Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  5. Edman P., Henschen A. 1975; Sequence determination. In Protein Sequence Determination, 2nd edn.. pp. 232–279 Needleman S. B. Edited by Berlin: Springer Verlag;
    [Google Scholar]
  6. French D. 1957; The schardingerdextrins. Advances in Carbohydrate Chemistry 12:189–260
    [Google Scholar]
  7. Henikoff S. 1984; Unidirectional digestion with exonuclease III creates targeted breakpoints for DNA sequencing. Gene 28:351–359
    [Google Scholar]
  8. Honda H., Kudo T., Horikoshi K. 1985; Selective excretion of alkaline xylanase by Escherichia coli carrying pCX311. Agricultural and Biological Chemistry 49:3011–3015
    [Google Scholar]
  9. Kaneko T., Kato T., Nakamura N., Horikoshi K. 1987; Spectrophotometric determination of cyclization activity of beta-cyclodextrin-forming cyclomaltodextrin glucanotransferase. Journal of the Japanese Society of Starch Science 34:45–48
    [Google Scholar]
  10. Kato C., Kudo T., Watanabe K., Horikoshi K. 1983; Extracellular production of Bacillus penicillinase by Escherichia coli carrying pEAP2. European Journal of Applied Microbiology and Biotechnology 18:339–343
    [Google Scholar]
  11. Kato C., Kudo T., Watanabe K., Horikoshi K. 1985; Nucleotide sequence of the β-lactamase gene of alkalophilic Bacillus sp. strain 170. Journal of General Microbiology 131:3317–3324
    [Google Scholar]
  12. Kitahata S., Tsuyama N., Okada S. 1974; Purification and some properties of cyclodextrin glycosyltransferase from a strain of Bacillus species. Agricultural and Biological Chemistry 38:387–393
    [Google Scholar]
  13. Kobayashi S., Shibuya N., Young B.M., French D. 1984; The preparation of 6-O-alpha-gluco-pyranosylcyclohexaamylose. Carbohydrate Research 126:215–224
    [Google Scholar]
  14. Langer P.R., Waldrop A.A., Ward D.C. 1981; Enzymatic synthesis of biotin-labelled polynucleotides: novel nucleic acid affinity probes. Proceedings of the National Academy of Sciences of the United States of America 78:6633–6637
    [Google Scholar]
  15. Lederberg E.M., Cohen S.N. 1974; Transformation of Salmonella typhimurium by plasmid deoxyribonucleic acid. Journal of Bacteriology 119:1072–1074
    [Google Scholar]
  16. Maniatis T., Fritsch E.F., Sambrook J. 1982 Molecular Cloning: a Laboratory Manual pp. 68–69 Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  17. Mclaughlin J.R., Murray C.L., Rabinowitz J.C. 1981; UniqUfe features in the ribosome binding site sequence of the Gram-positive Staphylococcus aureus beta-lactamase gene. Journal of Biological Chemistry 256:11283–11291
    [Google Scholar]
  18. Messing J., Crea R., Seeburg P.H. 1981; A system for shotgun DNA sequencing. Nucleic Acids Research 9:309–321
    [Google Scholar]
  19. Moore S., Stein W.H. 1963; Chromatographic determination of amino acids by the use of automatic recording equipment. Methods in Enzymology 6:819–831
    [Google Scholar]
  20. Murphy N., Mcconnell D.J., Cantwell B.A. 1984; The DNA sequence of the gene and genetic control sites for the excreted Bacillus subtilis enzyme beta-glucanase. Nucleic Acids Research 12:5355–5367
    [Google Scholar]
  21. Murray C.L., Rabinowitz J.C. 1982; Nucleotide sequences of transcription and translation regions in Bacillus phage ɸ29 early genes. Journal of Biological Chemistry 257:1053–1062
    [Google Scholar]
  22. Nakamura N., Horikoshi K. 1976; Purification and properties of neutral-cyclodextrin glycosyltrans- ferase of an alkalophilic Bacillus sp. Agricultural and Biological Chemistry 40:1785–1791
    [Google Scholar]
  23. Okada S., Kitahata S. 1973; A study of cyclodextrin-forming enzymes. Proceedings of Amylase Symposium Osaka: 18:21–27
    [Google Scholar]
  24. Palva I., Pettersson R.F., Kalkkinen N., Lehtovaara P., Sarvas M., Söderlund H., Takkinen K., Kääriäinen L. 1981; Nucleotide sequence of the promoter and NH2-terminal signal peptide region of the alpha-amylase gene from Bacillus amyloliquefaciens. Gene 15:43–51
    [Google Scholar]
  25. Pulley O.A., French D. 1961; Studies on the schardinger dextrins. XI.The isolation of new schardinger dextrins. Biochemical and Biophysical Research Communications 5:11–15
    [Google Scholar]
  26. Saito H., Miura K. 1963; Preparation of transforming DNA by phenol treatment. Biochimica et biophysica acta 72:619–629
    [Google Scholar]
  27. Sanger F., Nicklen S., Coulson A.R. 1977; DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences of the United States of America 74:5463–5467
    [Google Scholar]
  28. Shiosaka M., Fumiya H. 1973; Cyclodextrinforming enzyme of B stearothermophilus. Proceed-ings of Amylase Symposium Osaka: 1843–50
    [Google Scholar]
  29. Southern E.M. 1975; Detection of specific sequences among DNA fragments separated by gel electrophoresis. Journal of Molecular Biology 98:503–517
    [Google Scholar]
  30. Spackman D.H., Stein W.H., Moore S. 1958; Automatic recording apparatus for use in the chromatography of amino acids. Analytical Chemistry 30:1190–1206
    [Google Scholar]
  31. Stephens M.A., Ortlepp S.A., Ollington J.F., Mcconnell D.J. 1984; Nucleotide sequence of the 5’ region of the Bacillus licheniformis alpha- amylase gene: comparison with the B. amyloliquefaciens gene. Journal of Bacteriology 158:369–372
    [Google Scholar]
  32. Takano T., Fukuda M., Momma M., Kobayashi S., Kainuma K., Yamane K. 1986; Molecular cloning, DNA nucleotide sequencing, and expression in Bacillus subtilis cells of the Bacillus macerans cyclodextrin glucanotransferase gene. Journal of Bacteriology 166:1118–1122
    [Google Scholar]
  33. Weber K., Pringle J.R., Osborn M. 1972; Measurement of molecular weights by electrophoresis on SDS-acrylamide gel. Methods in Enzymology 26:3–27
    [Google Scholar]
  34. Yamamoto M., Tanaka Y., Horikoshi K. 1972; Alkaline amylases of alkalophilic bacteria. Agricultural and Biological Chemistry 36:1819–1823
    [Google Scholar]
  35. Yang M., Galizzi A., Henner D. 1983; Nucleotide sequence of the amylase gene from Bacillus subtilis. Nucleic Acids Research 11:237–249
    [Google Scholar]
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