1887

Abstract

The biochemical basis for the antimicrobial effect of the carrot phytoalexin 6-methoxymellein (6-MM) was examined. At fungistatic concentrations 6-MM retarded the ability of to incorporate radioactive thymidine, uridine and leucine into biopolymers. When was incubated with 6-MM, 260-nm-absorbing materials and H-labelled compounds leaked from the cells. The inhibitory effects of 6-MM on cell growth and membrane functions were, however, reduced as the concentration of divalent metal cations added to the medium was increased. 6-MM interacted with multilamellar liposomes constituted from phosphatidylcholine, cholesterol and dicetyl phosphate, or from phosphatidylcholine only, resulting in the release of glucose trapped in these liposomes. These results suggest that 6-MM exerts its toxic effects on susceptible cells as a result of its interaction with their membranes and disturbance of membrane-associated functions.

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/content/journal/micro/10.1099/00221287-134-1-241
1988-01-01
2022-01-18
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References

  1. Amin M., Kurosaki F., Nishi A. 1986; Inhibition of cyclic nucleotide phosphodiesterase by carrot phytoalexin. Phytochemistry 25:2305–2307
    [Google Scholar]
  2. Amin M., Kurosaki F., Nishi A. 1987; Carrot phytoalexin inhibits Ca2+, calmodulin dependent protein phosphorylation in carrot cells. Phytochemistry 26:51–53
    [Google Scholar]
  3. Bangham A.D., Standish M.W., Watkins J.C. 1965; Diffusion of univalent ions across the lamellae of swollen phospholipid. Journal of Molecular Biology 13:238–252
    [Google Scholar]
  4. Bolard J. 1986; How do macrolide antibiotics affect cellular membrane properties?. Biochimica et biophysica acta 864:257–304
    [Google Scholar]
  5. Boydston R., Paxton J.D., Koeppe D.E. 1983; Glyceollin, a site-specific inhibitor of electron transport in isolated soybean mitochondria. Plant Physiology 72:151–155
    [Google Scholar]
  6. Coxon D.T., Curtis F.R., Price K.R., Levett G. 1973; Abnormal metabolites produced by Daucus carota roots under conditions of stress. Phytochemistry 12:1881–1885
    [Google Scholar]
  7. Dixon R.A., Key P.M. 1983; Phytoalexins: enzymology and molecular biology. Advances in Enzymology 55:1–69
    [Google Scholar]
  8. Fraser R.S.S., Creanor J. 1974; Rapid and selective inhibition of RNA synthesis in yeast by 8-hydroxyquinoline. European Journal of Biochemistry 46:67–72
    [Google Scholar]
  9. Hargreaves J.A. 1980; A possible mechanism for the phytotoxicity of the phytoalexin phaseollin. Physiological Plant Pathology 16:251–357
    [Google Scholar]
  10. Hsuchen C.-C., Feingold D.S. 1973; Polyene antibiotic action on lecithin liposomes. Effect of cholesterol and fatty acyl chains. Biochemical and Biophysical Research Communications 51:972–978
    [Google Scholar]
  11. Imai M., Inoue K., Nojima A. 1975; Effect of polymyxin B on liposomal membranes derived from Escherichia coli lipids. Biochimica et biophysicaacta 375:130–137
    [Google Scholar]
  12. Kaplan D.T., Keen N.T., Thomason I.J. 1980; Studies on the mode of action of glyceollin in soybean. Incompatibility to root knot nematode Meloidogyne incognita . Physiological Plant Pathology 16:319–325
    [Google Scholar]
  13. Kerridge D. 1986; Mode of action of clinically important antifungal drugs. Advances in Microbial Physiology 27:1–72
    [Google Scholar]
  14. Kotani H., Shinmyo A., Enatsu T. 1977; Killer toxin for sake yeast: properties and effects of adenosine 5′-diphosphate and calcium ion on killing action. Journal of Bacteriology 129:640–650
    [Google Scholar]
  15. Kurosaki F., Nishi A. 1983; Isolation and antimicrobial activity of the phytoalexin 6-methoxy-mellein from cultured carrot cells. Phytochemistry 22:669–672
    [Google Scholar]
  16. Kurosaki F., Matsui K., Nishi A. 1984; Production and metabolism of 6-methoxymellein in cultured carrot cells. Physiological Plant Pathology 25:313–321
    [Google Scholar]
  17. Oku N., Nojima S., Inoue K. 1980; Selective release of non-electrolytes from liposomes upon perturbation of bilayers by temperature change or polyene antibiotics. Biochimica et biophysica acta 595:277–290
    [Google Scholar]
  18. Smith D.R. 1982 In Phytoalexins pp. 218–252 Bailey J.A., Mansfield J.W. Edited by Glasgow & London:: Blackie & Son;
    [Google Scholar]
  19. Storm D.R., Roesenthal K.S., Swanson P.E. 1977; Polymyxin and related peptide antibiotics. Annual Review of Biochemistry 46:723–763
    [Google Scholar]
  20. Swamy K.H., Sirsi M., Rao G.R. 1974; Studies on the mechanism of action of miconazole: effect of miconazole on respiration and cell permeability of Candida albicans. Antimicrobial Agents and Chemotherapy 5:420–425
    [Google Scholar]
  21. Weinstein L.I., Albersheim P. 1983; Host-pathogen interactions. XXIII. The mechanism of the antibacterial action of glycinol, a pterocarpan phytoalexin synthesized by soybeans. Plant Physiology 72:557–563
    [Google Scholar]
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