SUMMARY: An internal 0.9 kb segment of insertion sequence IS was used as a probe to determine the distribution of IS in strains of and other Gram-negative bacteria. The insertion sequence was detected in 80% (12/15) of strains from different parts of the world. Its copy number ranged from one to at least eleven. The IS copies detected showed variation in their internal restriction sites and their degree of homology to the probe. IS was found in a variety of genomic restriction fragments, and was detected in plasmids, including the and megaplasmids of Other rhizobia found to contain IS were a strain of biovar and two isolates capable of nodulating both and It was also found in a diazotrophic soil bacterium isolated from the roots of wetland rice.


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