SUMMARY: strains lacking the terminus region of the chromosome (min 29-36) due to an IS-promoted deletion did not grow well in rich medium; they also did not grow on fumarate minimal medium because (min 35·7) is deleted. Strains with secondary mutations that partially suppress the deletion phenotype displayed healthier growth on rich medium and grew on minimal fumarate medium. These suppressor mutants had an IS insertion just upstream of the structural gene (min 93·4). A strain with a Tn insertion at this location was constructed and used to delete nonessential deletion mutants grew well on both rich and minimal fumarate media.


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