1887

Abstract

SUMMARY: A method for the production of protoplasts of strain N1-4080 utilizing lysozyme and penicillin G was optimized to yield about 100% protoplasts. Treatment of 10 cells left fewer than 10 that were viable and osmo-resistant. With a mutant strain deficient in autolytic activity, about 1% of the protoplasts regenerated walled cells, whereas most gave rise to stable L-form colonies. Addition of sodium polyanethole sulphonate to the medium improved the regeneration frequency by at least a factor of 10, probably by somehow inhibiting the residual autolytic activity of the strain.

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/content/journal/micro/10.1099/00221287-133-9-2595
1987-09-01
2019-10-15
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http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-133-9-2595
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