RT Journal Article SR Electronic(1) A1 ROGERS, H. J. A1 WRIGHT, G.YR 1987 T1 Early Changes in Bacterial Envelopes after Inhibition of Peptidoglycan Synthesis, as Shown by the Use of a Fluorescent Probe JF Microbiology, VO 133 IS 9 SP 2567 OP 2572 DO https://doi.org/10.1099/00221287-133-9-2567 PB Microbiology Society, SN 1465-2080, AB SUMMARY: The probe 8-anilino-1-naphthalene sulphonate (ANS) showed increasing fluorescence with Bacillus subtilis metC lyt-2 cells taken from exponentially growing cultures treated with antibiotics that inhibit cell-wall peptidoglycan synthesis. This increase was due to the probe reaching hydrophobic cell constituents, probably membranes, and started within 30 min of the addition of the antibiotics. This corresponded to the time at which membrane function had been shown to be damaged. The increased fluorescence of the cells with ANS persisted after removal of the antibiotics., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-133-9-2567