RT Journal Article SR Electronic(1) A1 MALIK, AFSHAN N. A1 VIVIAN, ALAN A1 TAYLOR, JOHN D.YR 1987 T1 Isolation and Partial Characterization of Three Classes of Mutant in Pseudomonas syringae Pathovar pisi with Altered Behaviour towards Their Host, Pisum sativum JF Microbiology, VO 133 IS 9 SP 2393 OP 2399 DO https://doi.org/10.1099/00221287-133-9-2393 PB Microbiology Society, SN 1465-2080, AB SUMMARY: Mating with Escherichia coli strain SM10 carrying the Tn5 vector pSUP2011 was used to mutagenize Pseudomonas syringae pv. pisi strain 299A. The resulting transconjugants were each tested by stem-inoculation into several pea (Pisum sativum) cultivars. Three classes of mutant, which probably resulted from insertion of part or all of RP4-2-Tc:: Mu into the genome of strain 299A, showed reduced virulence towards one or more pea cultivars. The single class I mutant was avirulent on all pea cultivars tested and had lost the ability to induce a hypersensitive response in tobacco (Nicotiana tabacum) cv. White Burley; the single class II mutant induced a hypersensitive response on all pea cultivars and tobacco; class III mutants showed reduced virulence towards pea cv. Early Onward, while remaining fully virulent towards other normally susceptible pea cultivars, and inducing a hypersensitive response in tobacco., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-133-9-2393