RT Journal Article SR Electronic(1) A1 OKADA, KENJI A1 MITSUYAMA, MASAO A1 MIAKE, SHUNJI A1 AMAKO, KAZUNOBUYR 1987 T1 Monoclonal Antibodies against the Haemolysin of Vibrio vulnificus JF Microbiology, VO 133 IS 8 SP 2279 OP 2284 DO https://doi.org/10.1099/00221287-133-8-2279 PB Microbiology Society, SN 1465-2080, AB SUMMARY: The extracellular haemolysin produced by Vibrio vulnificus strain FCC was partially purified from the culture supernate by sequential ammonium sulphate precipitation, gel filtration with Sepharose 4B, and DEAE-Sephacel ion-exchange column chromatography. Using this semi-purified haemolysin as the antigen, several monoclonal antibodies (MAbs) were established; they were all of the IgG2b class with lambda light chains. One representative MAb, 6F8D, completely neutralized the haemolytic activity and mouse lethal activity of extracellular toxin(s). In immunoblotting analysis of the peptides of the semi-purified haemolysin separated by SDS-PAGE, this MAb reacted, in particular, with a 36 kDa peptide. These findings suggest that the haemolysin is probably identical to the lethal toxin in the culture supernate of V. vulnificus strain FCC, which contained the 36 kDa peptide., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-133-8-2279