1887

Abstract

SUMMARY: We have investigated the immunity to E colicins conferred by three group A klebicin plasmids. pP5a, which encodes klebicin Al-P5, like pClo-DF13, confers immunity to colicin E6 on K12, whilst pP5b and pP3, which encode klebicins A2-P5 and A3-P3 respectively, both confer immunity to colicin E3. We have determined the restriction endonuclease and functional maps of the three group A klebicin plasmids. By sub-cloning and transposon mutagenesis we have investigated the relationship between the klebicin immunity and the E colicin immunity conferred by these plasmids. The colicin E6 and the klebicin A1 immunity are encoded by a single gene present on pP5a. The colicin E3 and the klebicin A2 immunity are encoded by a single gene present on pP5b. The colicin E3 and the klebicin A3 immunity are encoded by separate genes present on pP3. Recombinant pML8412, which is derived from the ColE6-CT14 plasmid and encodes colicin E6 immunity, confers klebicin Al-P5 immunity upon UNF5023. Recombinant pKC23, which is derived from the ColE3-CA38 plasmid and confers colicin E3 immunity, confers immunity to klebicin A2-P5, but not to klebicin A3-P3.

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/content/journal/micro/10.1099/00221287-133-8-2253
1987-08-01
2019-11-20
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http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-133-8-2253
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