SUMMARY: was unable to grow in media containing above about 1·5 m free sulphite at pH 4·0, whereas grew at the same pH value in the presence of 7·8 m free sulphite. Expressed in terms of μl of intracellular water, the initial velocity of sulphite accumulation by was approximately twice that of , although the former yeast accumulated at equilibrium only about one-third of the amount of sulphite accumulated by . A Woolf-Hofstee plot for accumulation of SO by at pH 3·0 and 30°C gave a vertical line. Incorporation of sulphite in growth media induced excretion of acetaldehyde by both yeasts, the rate being greater by than . Acetaldehyde excretion was accompanied by release of lower concentrations of pyruvate. Excretion of 2-oxoglutarate was barely detectable. It is suggested that the greater resistance of to sulphite, compared with , may be explained partly by its decreased capacity to accumulate the compound, and partly by its ability to produce more acetaldehyde.


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