A Rapid and Efficient Method for Plasmid Transformation of and Free

Abstract

SUMMARY: A rapid and efficient method for plasmid transformation of M5al and K12 has been developed. The method, which uses a freeze-thaw cycle in the presence of CaCl to facilitate DNA uptake, is substantially more efficient for M5al than the conventional transformation procedure for . The simplicity and speed of the method makes it very attractive for routine transformation of M5al and K12.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-133-8-2053
1987-08-01
2024-03-28
Loading full text...

Full text loading...

http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-133-8-2053
Loading

Most cited Most Cited RSS feed