RT Journal Article SR Electronic(1) A1 COUSENS, DIANE J. A1 CLARKE, PATRICIA H. A1 DREW, ROBERTYR 1987 T1 The Amidase Regulatory Gene (amiR) of Pseudomonas aeruginosa JF Microbiology, VO 133 IS 8 SP 2041 OP 2052 DO https://doi.org/10.1099/00221287-133-8-2041 PB Microbiology Society, SN 1465-2080, AB SUMMARY: Recombinant plasmids carrying the amidase genes of Pseudomonas aeruginosa were used to study the genetic control of amidase synthesis in Escherichia coli and Pseudomonas aeruginosa. The amidase regulator gene, amiR, was found to lie about 2 kbp downstream from the structural gene, amiE. Using plasmids with in vitro-constructed deletions, and plasmids containing sub-cloned DNA fragments, the amiR gene was located within a l kbp ClaI-XhoI DNA fragment. The structural and regulator genes were shown to be transcribed in the same direction. Deletion of DNA sequences between the two genes resulted in increased synthesis of amidase in both E. coli and P. aeruginosa. The intervening sequences showed no repressing effect when tested in trans. The results suggested that the amiR gene could be transcribed from more than one promoter., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-133-8-2041