SUMMARY: Recombinant plasmids carrying the amidase genes of were used to study the genetic control of amidase synthesis in and The amidase regulator gene, , was found to lie about 2 kbp downstream from the structural gene, Using plasmids with -constructed deletions, and plasmids containing sub-cloned DNA fragments, the gene was located within a l kbp I-I DNA fragment. The structural and regulator genes were shown to be transcribed in the same direction. Deletion of DNA sequences between the two genes resulted in increased synthesis of amidase in both and . The intervening sequences showed no repressing effect when tested in The results suggested that the gene could be transcribed from more than one promoter.


Article metrics loading...

Loading full text...

Full text loading...


Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error