1887

Abstract

SUMMARY: Recombinant plasmids carrying the amidase genes of were used to study the genetic control of amidase synthesis in and The amidase regulator gene, , was found to lie about 2 kbp downstream from the structural gene, Using plasmids with -constructed deletions, and plasmids containing sub-cloned DNA fragments, the gene was located within a l kbp I-I DNA fragment. The structural and regulator genes were shown to be transcribed in the same direction. Deletion of DNA sequences between the two genes resulted in increased synthesis of amidase in both and . The intervening sequences showed no repressing effect when tested in The results suggested that the gene could be transcribed from more than one promoter.

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/content/journal/micro/10.1099/00221287-133-8-2041
1987-08-01
2024-11-08
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/content/journal/micro/10.1099/00221287-133-8-2041
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