SUMMARY: Xanthine oxidase with acetaldehyde as substrate (the XOA system) generated superoxide anion and hydrogen peroxide, but this system had only weak bactericidal activity. Addition of Fe and EDTA to the XOA system (XOA-Fe-EDTA system) increased bactericidal activity against and , although both and remained highly resistant. Catalase (HO scavenger) and mannitol (OH scavenger) almost completely inhibited the bactericidal activity of the XOA-Fe-EDTA system whereas SOD (O scavenger) was less inhibitory, Azide (O scavenger) caused no such inhibition. The results suggest the possible role of OH, HO and O in the XOA-Fe-EDTA-mediated antimicrobial system, as effector molecules. There was no correlation between resistance of a given bacterium to active oxygen and the level of endogenous active oxygen-scavengers.


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