SUMMARY: A bacterium capable of growth on -2-butene was isolated from soil and identified as a sp. The isolate also grew on propane, butane, pentane and hexane; gaseous and volatile 1-alkenes did not support growth. Intact cells grown on -2-butene or butane oxidized all n-alkanes, 1-alkenes, 2-alkenes and alcohols tested. Simultaneous adaptation studies, inhibitor experiments and measurements of enzyme activities in crude extracts indicated a degradative route of -2-butene via crotonic acid and of butane via butyric acid. The key enzymes in the proposed pathways were induced by growth on either -2-butene or butane. The induction of these enzymes and the substrate specificities of the enzymes suggest a relation between -2-butene and butane degradation.


Article metrics loading...

Loading full text...

Full text loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error