1887

Abstract

SUMMARY: A bacterium capable of growth on -2-butene was isolated from soil and identified as a sp. The isolate also grew on propane, butane, pentane and hexane; gaseous and volatile 1-alkenes did not support growth. Intact cells grown on -2-butene or butane oxidized all n-alkanes, 1-alkenes, 2-alkenes and alcohols tested. Simultaneous adaptation studies, inhibitor experiments and measurements of enzyme activities in crude extracts indicated a degradative route of -2-butene via crotonic acid and of butane via butyric acid. The key enzymes in the proposed pathways were induced by growth on either -2-butene or butane. The induction of these enzymes and the substrate specificities of the enzymes suggest a relation between -2-butene and butane degradation.

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/content/journal/micro/10.1099/00221287-133-7-1713
1987-07-01
2019-10-21
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http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-133-7-1713
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