%0 Journal Article %A Markevics, Lynda J. %A Kah, Kim K. %A Rathsam, Leander %A Turner, Laurence W. %A Jacques, Nicholas A. %T Adaptation of the Membrane Fatty Acid Composition by Growth in the Presence of n-Alkanols Influences Glycosyltransferase Expression in Streptococcus salivarius %D 1987 %J Microbiology, %V 133 %N 6 %P 1543-1551 %@ 1465-2080 %R https://doi.org/10.1099/00221287-133-6-1543 %I Microbiology Society, %X Summary: Growth of Streptococcus salivarius ATCC 25975 in the presence of n-alkanols in the series methanol to decan-1-ol led to a decrease in the unsaturated to saturated fatty acid ratio. Each member of the set of n-alkanols which was examined over a range of concentrations possessed a point at which extracellular glucosyltransferase (GTF) production was minimal; increasing the concentration of the n-alkanol past this point stimulated GTF production. This effect was greatest with hexan-1 -ol although it was observed to a lesser extent with pentan-1 -ol and heptan-1-ol. Reduced cell-associated fructosyltransferase activity was observed with increasing concentrations of each n-alkanol. Growth in the presence of 25 mM-propan-1-ol gave rise to a fatty acid profile in which 55% of the fatty acids were of an odd chain length. S. salivarius ATCC 25975 was shown to be able to utilize ethanol in a similar manner to propan-1-ol by growing it in the presence of 400 mM-[14C]ethanol. Analysis of the membrane lipids at the stationary phase of growth indicated that 17ยท6 % of the carbon of the fatty acids was derived from ethanol. A leaky adh mutant, S. salivarius MJ 37501, was isolated. The leaky nature of the mutant enabled it to incorporate reduced levels of odd-chain-length fatty acids into its membrane lipids when grown in the presence of 100 mM-propan-1-ol, but not when grown in the presence of 25 mM-propan-1-ol. S. salivarius ATCC 25975 therefore metabolized propan-1-ol (and ethanol) via a constitutive alcohol dehydrogenase. %U https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-133-6-1543