1887

Abstract

SUMMARY: strain AHT (serotype ) secretes at least two glucosyltransferases with different pI values. A novel glucosyltransferase with a pI of 5·8 was purified 244-fold from the ammonium sulphate fraction by DEAE-cellulose chromatography, FPLC (Mono Q column, Pharmacia) and hydrophobic chromatography. The enzyme preparation gave a single protein band on analysis by both PAGE and SDS-PAGE, and did not form multiple protein bands detectable by IEF. The was estimated to be about 130000 by SDS-PAGE and about 135000 by ultracentrifugal analysis. The apparent value and pH optimum of the enzyme were 3·9 ± 0·2 m (mean ± ) and about 4·7, respectively. The enzyme synthesized water-soluble glucan from sucrose, and the glucan consisted of over 90 mol% 1,6-α--glucosidic linkages. The enzyme activity was not stimulated by primer dextran. Anti-enzyme serum produced a single precipitin band with the purified enzyme preparation, whereas it did not react with either of the other two known glucosyltransferases.

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/content/journal/micro/10.1099/00221287-133-5-1351
1987-05-01
2022-01-29
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