1887

Abstract

A glycolipid antigen possessing a serovar-specific antigenic determinant of serovar was purified from a chloroform/methanol extract of the organism. The purification procedures included silicic acid column chromatography and preparative thin-layer chromatography (TLC). Antigenic activity was detected by a TLC-enzyme immunostaining technique using monoclonal antibody CT3, which specifically agglutinates serovar and only weakly serovar but no other serovars of . The purified glycolipid reacted with CT3 antibody, indicating that the glycolipid possessed a serovar-specific antigenic determinant. Infrared spectrum and proton nuclear magnetic resonance analyses showed that the glycolipid contained sugar and lipid moieties, which possessed amide linkages and an acetyl group. Gas-liquid chromatography-mass spectrometry analysis showed that the glycolipid contained two unknown sugars, one of which (unknown sugar II) appeared to be associated with the antigenic determinant specific for . The serovar-specific antigenic determinant was destroyed by mild alkali treatment of the glycolipid. These findings suggested that the antigenic determinant was an alkali-labile moiety which may be related to the unknown sugar II.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-133-5-1329
1987-05-01
2021-10-28
Loading full text...

Full text loading...

/deliver/fulltext/micro/133/5/mic-133-5-1329.html?itemId=/content/journal/micro/10.1099/00221287-133-5-1329&mimeType=html&fmt=ahah

References

  1. Adachi Y., Yanagawa R. 1975; Possible role of protein(s) as antigenic determinant of the type-specific main antigen of Leptospira kremastos strain Kyoto. Infection and Immunity 11:1325–1331
    [Google Scholar]
  2. Adachi Y., Yanagawa R. 1977; Inhibition of leptospiral agglutination by the type-specific main antigen of leptospiras. Infection and Immunity 17:466–467
    [Google Scholar]
  3. Adachi Y., Yanagawa R. 1978; Nature of antigenic determinant of serovar-specific antigen of Leptospira interrogans serovar hebdomadis. Microbiology and Immunology 22:523–533
    [Google Scholar]
  4. Bey R. F., Johnson R. C. 1978; Protein-free and low-protein media for the cultivation of Leptospira. Infection and Immunity 19:562–569
    [Google Scholar]
  5. Brennan P. J., Goren M. B. 1979; Structural studies on the type-specific antigens and lipids of the Mycobacterium avium-Mycobacterium intracellulare-Mycobacterium scrofulaceum serocomplex. Journal of Biological Chemistry 254:4205–4211
    [Google Scholar]
  6. Brennan P. J., Aspinall G. O., Shin J. E. 1981; Structure of the specific oligosaccharides from the glycopeptidolipid antigen of serovars in the Mycobacterium avium-Mycobacterium intracellulare-Mycobacterium scrofulaceum complex. Journal of Biological Chemistry 256:6817–6822
    [Google Scholar]
  7. Cox C. D. 1955; Hemolysis of sheep erythrocytes sensitized with leptospiral extracts. Proceedings of the Society for Experimental Biology and Medicine 90:610–615
    [Google Scholar]
  8. Dikken H., Kmety E. 1978; Serological typing methods of leptospires. Methods in Microbiology 11:260–308
    [Google Scholar]
  9. Hawkes R., Niday E., Gordon J. 1982; A dot-immunobinding assay for monoclonal and other antibodies. Analytical Biochemistry 119:142–147
    [Google Scholar]
  10. Heller S. R., Milne G. W. A. 1978 EPA/NIH Mass Spectral Data Base Washington: US Government Printing Office;
    [Google Scholar]
  11. Higashi H., Fukui Y., Ueda S., Kato S., Hirabayashi Y., Matsumoto M., Naiki M. 1984; Sensitive enzyme-immunostaining and densitome-tric determination on thin-layer chromatography of N-glycolylneuraminic acid-containing glycosphingo-lipids, Hanganutziu-Deicher antigens. Journal of Biochemistry 95:1517–1520
    [Google Scholar]
  12. Hunter S. W., Fujiwara T., Brennan P. J. 1982; Structure and antigenicity of the major specific glycolipid antigen of Mycobacterium leprae. Journal of Biological Chemistry 257:15072–15078
    [Google Scholar]
  13. Hunter S. W., Jardine I., Yanagihara D. L., Brennan P. J. 1985; Trehalose-containing lipo-oligosaccharides from mycobacteria: structure of the oligosaccharide segments and recognition of a unique A-acylkanosamine-containing epitope. Biochemistry 24:2798–2805
    [Google Scholar]
  14. Johnson R. C., Livermore P. B., Walby J. K., Jenkin H. M. 1970; Lipids of parasitic and saprophytic leptospires. Infection and Immunity 2:286–291
    [Google Scholar]
  15. Kasai N., Yanagawa R. 1974; Studies on the antigenic determinant group of the type-specific antigen of Leptospira canicola. Zentralblatt für Bakteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene (Abteilung I, Originate A) 228:533–541
    [Google Scholar]
  16. Kawaoka Y., Naiki M., Yanagawa R. 1979; Radioimmunoassay system using a serovar-specific lipopolysaccharide antigen of Leptospira. Journal of Clinical Microbiology 10:313–316
    [Google Scholar]
  17. Kawaoka Y., Naiki M., Yanagawa R. 1982; Isolation of antigen-active components from leptospiral serovar-specific lipopolysaccharide antigen by mild acid hydrolysis. Japanese Journal of Veterinary Science 44:473–478
    [Google Scholar]
  18. Naiki M., Fong J., Ledeen R., Marcus D. M. 1975; The structure of the human erythrocyte blood group P, glycosphingolipid. Biochemistry 14:4831–4836
    [Google Scholar]
  19. Ono E., Naiki M., Yanagawa R. 1982; Production and characterization of monoclonal antibodies to lipopolysaccharide antigen of Leptospira interrogans serovar kremastos and canicola. Zentralblatt für Bakteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene (Abteilung I, Originate A) 252:414–424
    [Google Scholar]
  20. Ono E., Naiki M., Yanagawa R. 1984; Isolation of an antigenic oligosaccharide fraction from Leptospira interrogans serovar canicola with a monoclonal antibody. Journal of General Microbiology 130:1429–1435
    [Google Scholar]
  21. Shimono E., Sugiyama K., Yanagawa R. 1979; Specificity of serovar-specific main antigen of leptospiras shown by the inhibition of leptospiral microscopic agglutination. Japanese Journal of Veterinary Science 41:623–628
    [Google Scholar]
  22. Shinagawa M., Yanagawa R. 1972; Isolation and characterization of a leptospiral type-specific antigen. Infection and Immunity 5:12–19
    [Google Scholar]
  23. Tsuji M., Kawaoka Y., Naiki M., Yanagawa R. 1981; Isolation of antigenically active components from leptospiral serovar-specific lipopolysaccharide antigen by alkaline treatment. Microbiology and Immunology 25:949–957
    [Google Scholar]
  24. Yanagawa R., Shinagawa M., Takashima I. 1974; Serological studies of leptospiras by immunodiffusion. Zentralblatt für Bakteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene (Abteilung I, Originate A) 228:369–377
    [Google Scholar]
  25. Yanagihara Y., Kamisango K., Yasuda S., Kobayashi S., Mlfuchi I., Azuma I., Yamamura Y., Johnson R. C. 1984; Chemical composition of cell walls and polysaccharide fractions of spirochetes. Microbiology and Immunology 28:535–544
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-133-5-1329
Loading
/content/journal/micro/10.1099/00221287-133-5-1329
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error