1887

Abstract

Phages coding for production of Vero cytotoxins VT1 or VT2 in strains of serotype O157. H7 or O157. H were morphologically indistinguishable. Their genome size and restriction enzyme digests of the phage DNA were similar. These phages were clearly different in these respects from a VT1-encoding phage isolated from a strain of O26. H11 (H19). However the VT1 region cloned from the phage originating in the O157. H7 strain was identical to the VT1 region previously cloned from the phage carried by H19. Sequences encoding VT2 that were cloned from the phage in O157. H have been mapped and the VT2 region identified by transposon insertion. The cloned regions coding for VT1 or VT2 production had no similarities in the presence of restriction enzyme sites over a distance of about 2 kb, and two VT1-specific probes spanning a region of about 1·4 kb did not hybridize under stringent conditions with cloned VT2 DNA. A 2 kb cII fragment contained the VT2 genes but hybridized to VT1-encoding phages and recombinant plasmids via flanking phage DNA. A 0·85 kb I-I fragment was a specific probe for VT2 sequences and did not hybridize under stringent conditions to phages or plasmid recombinants encoding VT1.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-133-5-1309
1987-05-01
2021-08-04
Loading full text...

Full text loading...

/deliver/fulltext/micro/133/5/mic-133-5-1309.html?itemId=/content/journal/micro/10.1099/00221287-133-5-1309&mimeType=html&fmt=ahah

References

  1. Bachmann B. J. 1972; Pedigrees of some mutant strains of Escherichia coli K12. Bacteriological Reviews 36:525–557
    [Google Scholar]
  2. Birnboim H. C., Doly J. 1979; A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Research 7:1513–1523
    [Google Scholar]
  3. Chang A. C. Y., Cohen S. N. 1978; Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid. Journal of Bacteriology 134:1141–1156
    [Google Scholar]
  4. Gulley P. R. 1984; Haemolytic-uraemic syndrome: epidemiology and report of an outbreak. Journal of the Royal Society of Health 1984 104:214–217
    [Google Scholar]
  5. Guyer M. S. 1978; The λδ sequence of F is an insertion sequence. Journal of Molecular Biology 126:347–365
    [Google Scholar]
  6. Huang A., Degrandis S., Friesen J., Karmali M., Petric M., Congi R., Brunton J. L. 1986; Cloning and expression of the genes specifying Shiga-like toxin production in Escherichia coli H19. Journal of Bacteriology 106:375–379
    [Google Scholar]
  7. Humphreys G. O., Willshaw G. A., Anderson E. S. 1975; A simple method for the preparation of large quantities of pure plasmid DNA. Biochimica et biophysica acta 383:457–463
    [Google Scholar]
  8. Johnson W. M., Lior H., Bezanson G. S. 1983; Cytotoxic Escherichia coli O157 : H7 associated with haemorrhagic colitis in Canada. Lancet i:76
    [Google Scholar]
  9. Karmali M. A., Steele B. T., Petric M., Lim C. 1983; Sporadic cases of haemolytic uraemic syndrome associated with faecal cytotoxin and cyto-toxin-producing Escherichia coliIn stools. Lancet i:619–620
    [Google Scholar]
  10. Konowalchuk J., Speirs J. I., Stavric S. 1977; Vero response to a cytotoxin of Escherichia coli. Infection and Immunity 18:775–779
    [Google Scholar]
  11. Maniatis T., Fritsch E. F., Sambrook J. 1982 Molecular Cloning: a Laboratory Manual Cold Spring Harbor, NY:: Cold Spring Harbor Laboratory.;
    [Google Scholar]
  12. Newland J. W., Strockbine N. A., Miller S. F., O’brien A. D., Holmes R. K. 1985; Cloning of Shiga-like toxin structural genes from a toxin converting phage of Escherichia coli. Science 230:179–181
    [Google Scholar]
  13. O’brien A. D., Laveck G. D. 1983; Purification and characterization of a Shigella dysenteriae 1-like toxin produced by Escherichia coli. Infection and Immunity 40:675–683
    [Google Scholar]
  14. O’brien A. D., La veck G. D., Thompson M. R., Formal S. B. 1982; Production of Shigella dysenteriae type 1-like cytotoxin by Escherichia coli. Journal of Infectious Diseases 146:763–769
    [Google Scholar]
  15. O’brien A. D., Newland J. W., Miller S. F., Holmes R. K., Smith H., Williams & Formal S. 1984; Shiga-like toxin-converting phages from Escherichia coli strains that cause hemorrhagic colitis or infantile diarrhea. Science 226:694–696
    [Google Scholar]
  16. Olsnes S., Eiklid K. 1980; Isolation and characterization of Shigella shigae cytotoxin. Journal of Biological Chemistry 255:284–289
    [Google Scholar]
  17. Riley L. W., Remis R. S., Helgerson S. D., Mcgee H. B., Wells J. G., Davis B. R., Hebert R. J., Olcott E. S., Johnson L. M., Hargrett N. T., Blake P. A., Cohen M. L. 1983; Hemorrhagic colitis associated with a rare Escherichia coli serotype. New England Journal of Medicine 308:681–685
    [Google Scholar]
  18. Scotland S. M., Day N. P., Rowe B. 1980; Production of a cytotoxin affecting Vero cells by strains of Escherichia coli belonging to traditional enteropathogenic serogroups. FEMS Microbiology Letters 7:15–17
    [Google Scholar]
  19. Scotland S. M., Smith H. R., Willshaw G. A. 1983; Vero cytotoxin production in strain of Escherichia coli is determined by genes carried on bacteriophage. Lancet ii:216
    [Google Scholar]
  20. Scotland S. M., Smith H. R., Rowe B. 1985; Two distinct toxins active on Vero cells from Escherichia coli O157. Lancet ii:885–886
    [Google Scholar]
  21. Scotland S. M., Rowe B., Smith H. R., Willshaw G. A., Gross R. J. 1987; Vero cytotoxin producing strains of Escherichia coli from children with haemolytic uraemic syndrome and their detection by specific DNA probes. Journal of Medical Microbiology (in the Press)
    [Google Scholar]
  22. Smith H. R., Day N. P., Scotland S. M., Gross R. J., Rowe B. 1984; Phage-determined production of Vero cytotoxin in strains of Escherichia coli serogroup O157. Lancet i:1242–1243
    [Google Scholar]
  23. Smith Williams H., Green P., Parsell Z. 1983; Vero cell toxins in Escherichia coli and related bacteria: transfer by phage and conjugation and toxic action in laboratory animals, chickens and pigs. Journal of General Microbiology 129:3112–3137
    [Google Scholar]
  24. Southern E. M. 1975; Detection of specific sequences among DNA fragments separated by agarose gel electrophoresis. Journal of Molecular Biology 98:503–517
    [Google Scholar]
  25. Strockbine N., Marques L. R. M., Newland J. W., Smith Williams H., Homes R. K., O’brien A. D. 1986; Two toxin-converting phages from Escherichia coli O157 : H7 strain 933 encode antigenically distinct toxins with similar biologic activities. Infection and Immunity 53:135–140
    [Google Scholar]
  26. Taylor C. M., White R. H. R., Winterborn M. H., Rowe B. 1986; Haemolytic-uraemic syndrome: clinical experience of an outbreak in the West Midlands. British Medical Journal 292:1513–1516
    [Google Scholar]
  27. Wade W. G., Thom B. T., Evans N. 1979; Cytotoxic enteropathogenic Escherichiu coli. Lancet ii:1235–1236
    [Google Scholar]
  28. Willshaw G. A., Smith H. R., Scotland S. M., Rowe B. 1985; Cloning of genes determining the production of Vero cytotoxin by Escherichia coli. Journal of General Microbiology 131:3047–3053
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-133-5-1309
Loading
/content/journal/micro/10.1099/00221287-133-5-1309
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error