RT Journal Article SR Electronic(1) A1 Kusnan, Misri B. A1 Berger, Michael G. A1 Fock, Heinrich P.YR 1987 T1 The Involvement of Glutamine Synthetase/Glutamate Synthase in Ammonia Assimilation by Aspergillus Nidulans JF Microbiology, VO 133 IS 5 SP 1235 OP 1242 DO https://doi.org/10.1099/00221287-133-5-1235 PB Microbiology Society, SN 1465-2080, AB Wild-type Aspergillus nidulans grew equally well on NH4Cl, KNO3 or glutamine as the only nitrogen source. NADP+-dependent glutamate dehydrogenase (EC 1.4.1.4) and glutamine synthetase (GS; EC 6.3.1.2) activities varied with the type and concentration of nitrogen source supplied. Glutamate synthase (GOGAT) activity (EC 1.4.7.1) was detected but it was almost unaffected by the type and concentration of nitrogen source supplied. Ion exchange chromatography showed that the GOGAT activity was due to a distinct enzyme. Azaserine, an inhibitor of the GOGAT reaction, reduced the glutamate pool by 60%, indicating that GOGAT is involved in ammonia assimilation by metabolizing the glutamine formed by GS., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-133-5-1235