1887

Abstract

The thermostability of the respiratory chain of grown at different temperatures was similar to the thermotolerance of the primary dehydrogenases. NADH was the major electron donor to the respiratory chain in cells grown at both 37 °C and 55 °C. The respiratory chain from cells grown at 55 °C exhibited slightly greater thermostability than that from cells grown at 37 °C. NADH-supported respiration, as well as NADH dehydrogenase activity, was much more thermotolerant than that with succinate in cells grown at both temperatures. Membrane-bound succinate dehydrogenase could be stabilized by the addition of 10% (w/v) NaCl while NADH dehydrogenase exhibited intrinsic thermostability.

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1987-05-01
2024-11-08
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References

  1. Amelunxen R. E., Murdock A. L. 1978; Mechanisms of thermophily. CRC Critical Reviews in Microbiology 6:343–393
    [Google Scholar]
  2. Ball A. S., Edwards C. 1986; The respiratory chain of the facultative thermophile Bacillus coagu- lans. Archives of Microbiology 145:347–352
    [Google Scholar]
  3. Ball A., Edwards C., Jones M. V. 1985; .Properties of the ATP phosphohydrolase of the facultative thermophile Bacillus coagulans. FEMS Microbiology Letters 27:139–142
    [Google Scholar]
  4. Bernath P., Singer T. P. 1962; Succinic dehydrogenase. Methods in Enzymology 5:597–617
    [Google Scholar]
  5. Crabb J. W., Murdock A. L., Amelunxen R. E. 1975; A proposed mechanism of thermophily in facultative thermophiles. Biochemical and Biophysical Research Communications 62:627–633
    [Google Scholar]
  6. Damadian R. 1973; Biological ion exchange resins. Annals of the New York Academy of Sciences 204:211–234
    [Google Scholar]
  7. Edwards C., Jones M. V. 1983; Production of a thermostable ATPase by the facultative thermophile Bacillus coagulans. Archives of Microbiology 135:74–76
    [Google Scholar]
  8. Galante Y. M., Hatefi Y. 1978; Resolution of complex 1 and isolation of NADH dehydrogenase and an iron-sulphur protein. Methods in Enzymology 53:15–22
    [Google Scholar]
  9. Heinen W., Lauwers A. M. 1983; Changes in enzyme stability and fatty acid composition of Streptomycessp., a facultative thermophile actino- mycete. Archives of Microbiology 134:247–250
    [Google Scholar]
  10. Jones M. V., Spencer W. 1985; Thermostability of enzymes of the tricarboxylic acid cycle of Bacillus coagulans. Antonie van Leeuwenhoek 51:193–201
    [Google Scholar]
  11. Jones M. V., Spencer W. N., Edwards C. 1984; Temperature dependent azide sensitivity of growth and ATPase activity in the facultative thermophile Bacillus coagulans. Journal of General Microbiology 130:95–101
    [Google Scholar]
  12. Lauwers A. M., Heinen W., Mulders J. W. M. 1981; Properties of enzymes from bacteria grown in the 70 °C-100 °C range. Archives of Microbiology 130:159–164
    [Google Scholar]
  13. Sharp R. J., Brown K. J., Atkinson A. 1980; Phenotypic and genotypic characterization of some thermophilic species of Bacillus. Journal of General Microbiology 117:201–210
    [Google Scholar]
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