SUMMARY: The role of fructose 2,6-bisphosphate in the regulation of glycolysis in the citric acid accumulating fungus was investigated. Fructose 2,6-bisphosphate stimulated the activity of partially purified phosphofructokinase by increasing the affinity of the enzyme for fructose 6-phosphate and relieving inhibition by ATP. Fructose 2,6-bisphosphate acted synergistically with AMP, but not with NH ions, which otherwise also activate phosphofructokinase. Fructose 2,6-bisphosphate also partially antagonized citrate inhibition of phosphofructokinase; complete deinhibition against high (5 mM) concentrations of citrate (as occur during citric acid accumulation), however, required the simultaneous presence of fructose 2,6-bisphosphate (0·1 μM). AMP (0·1 μM) and NH ions (20 mM).


Article metrics loading...

Loading full text...

Full text loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error