1887

Abstract

The role of fructose 2,6-bisphosphate in the regulation of glycolysis in the citric acid accumulating fungus was investigated. Fructose 2,6-bisphosphate stimulated the activity of partially purified phosphofructokinase by increasing the affinity of the enzyme for fructose 6-phosphate and relieving inhibition by ATP. Fructose 2,6-bisphosphate acted synergistically with AMP, but not with NH ions, which otherwise also activate phosphofructokinase. Fructose 2,6-bisphosphate also partially antagonized citrate inhibition of phosphofructokinase; complete deinhibition against high (5 m) concentrations of citrate (as occur during citric acid accumulation), however, required the simultaneous presence of fructose 2,6-bisphosphate (0·1 μ). AMP (0·1 μ) and NH ions (20 m).

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/content/journal/micro/10.1099/00221287-133-5-1195
1987-05-01
2022-01-21
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References

  1. Bartrons R., Van Schaftingen E., Vissers S., Hers H.-G. 1982; The stimulation of yeast phosphofructokinase by fructose 2,6-bisphosphate. FEBS Letters 143:137–140
    [Google Scholar]
  2. Habison A., Kubicek C. P., Röhr M. 1979; Phosphofructokinase as a regulatory enzyme in citric acid producing Aspergillus niger. FEMS Microbiology Letters 5:39–42
    [Google Scholar]
  3. Habison A., Kubicek C. P., Röhr M. 1983; Partial purification and regulatory properties of phosphofructokinase from Aspergillus niger. Biochemical Journal 209:669–676
    [Google Scholar]
  4. Hers H.-G., Van Schaftingen E. 1982; Fructose-2, 6-bisphosphate two years after its discovery. Biochemical Journal 206:1–12
    [Google Scholar]
  5. Heylen A., Van Schaftingen E., Hers H.-G. 1982; The stimulation of phosphofructokinase from human erythrocytes by fructose 2,6 bisphosphate. FEBS Letters 143:141–143
    [Google Scholar]
  6. Kubicek C. P., Röhr M. 1978; The role of the tricarboxylic acid cycle in citric acid accumulation by Aspergillus niger. European Journal of Applied Microbiology and Biotechnology 5:263–271
    [Google Scholar]
  7. Kubicek C. P., Röhr M. 1986; The citric acid fermentation. CRC Critical Reviews in Biotechnology 3:331–371
    [Google Scholar]
  8. Kubicek C. P., Hampel W., Röhr M. 1979; Manganese deficiency leads to elevated amino acid pool levels in citric acid accumulating Aspergillus niger. Archives of Microbiology 123:73–79
    [Google Scholar]
  9. Meixner-Monori B., Kubicek C. P., Röhr M. 1984; Pyruvate kinase from Aspergillus niger: a regulatory enzyme in glycolysis?. Canadian Journal of Microbiology 30:16–23
    [Google Scholar]
  10. Ogawa Y., Atkinson D. E. 1985; Interactions between citrate and nucleoside triphosphates in binding to phosphofructokinase. Biochemistry 24:954–958
    [Google Scholar]
  11. Röhr M., Kubicek C. P. 1981; Regulatory aspects of citric acid fermentation by Aspergillus niger. Process Biochemistry 16:34–37
    [Google Scholar]
  12. Schreferl G., Kubicek C. P., Röhr M. 1986; Inhibition of citric acid accumulation by manganese ions in Aspergillus niger mutants with reduced citrate control of phosphofructokinase. Journal of Bacteriology 165:1019–1022
    [Google Scholar]
  13. Uyeda K. 1979; Phosphofructokinase. Advances in Enzymology and Related Areas of Molecular Biology 48:193–244
    [Google Scholar]
  14. Uyeda K., Furuya E., Luby L. J. 1981; The effect of natural and synthetic d-fructose-2,6-bis- phosphate on the regulatory kinetic properties of liver and muscle phosphofructokinases. Journal of Biological Chemistry 256:8394–8399
    [Google Scholar]
  15. Van Laere A. J. 1983; Stimulation of phosphofructokinase from Phycomyces blakesleeanus and some other fungi by micromolar concentrations of fructose 2,6-bisphosphate. Journal of General Microbiology 129:3281–3285
    [Google Scholar]
  16. Van Schaftingen E., Hue L., Hers H.-G. 1980a; Control of the fructose 6-phosphate/fructose 1,6- bisphosphate cycle in isolated hepatocytes by glucose and glucagon. Biochemical Journal 192:887–895
    [Google Scholar]
  17. Van Schaftingen E., Hue L., Hers H.-G. 1980b; Fructose 2,6-bisphosphate, the probable structure of the glucose and glucagon sensitive stimulator of phosphofructokinase. Biochemical Journal 192:897–901
    [Google Scholar]
  18. Van Schaftingen E., Jett M.-F., Hue L., Hers H.-G. 1981; Control of liver 6-phosphofructokinase by fructose-2,6-bisphosphate and other effectors. Proceedings of the National Academy of Sciences of the United States of America 78:3483–3486
    [Google Scholar]
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