1887

Abstract

SUMMARY: The role of fructose 2,6-bisphosphate in the regulation of glycolysis in the citric acid accumulating fungus was investigated. Fructose 2,6-bisphosphate stimulated the activity of partially purified phosphofructokinase by increasing the affinity of the enzyme for fructose 6-phosphate and relieving inhibition by ATP. Fructose 2,6-bisphosphate acted synergistically with AMP, but not with NH ions, which otherwise also activate phosphofructokinase. Fructose 2,6-bisphosphate also partially antagonized citrate inhibition of phosphofructokinase; complete deinhibition against high (5 mM) concentrations of citrate (as occur during citric acid accumulation), however, required the simultaneous presence of fructose 2,6-bisphosphate (0·1 μM). AMP (0·1 μM) and NH ions (20 mM).

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/content/journal/micro/10.1099/00221287-133-5-1195
1987-05-01
2019-10-15
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http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-133-5-1195
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