1887

Abstract

A collection of 655 thermosensitive mutants of 168, obtained by indirect selection, was screened for those lysing at the non-permissive temperature. Thirty-three mutations thus identified were distributed by transformation into eight linkage groups designated to . The distribution was non-random. With the exception of group A, all groups were small, suggesting that mutations identified in each of them may map in one gene only. Linkage groups identified here were mapped in four different regions of the chromosome and their positions relative to reference markers were the following: (i) ; (ii) ; (iii) ; and (iv) . Kinetics of -acetyl-D-[1-C]glucosamine incorporation revealed that groups A, B, C, D and F are deficient in peptidoglycan synthesis at the restrictive temperature. In group G, anomalies at the cell wall level were suggested by incorporation and growth curves. It appears that in almost all known cases, thermosensitive lysis mutations in either affect genes involved in peptidoglycan synthesis or lead, more or less directly, to induction of prophages.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-133-5-1159
1987-05-01
2021-07-24
Loading full text...

Full text loading...

/deliver/fulltext/micro/133/5/mic-133-5-1159.html?itemId=/content/journal/micro/10.1099/00221287-133-5-1159&mimeType=html&fmt=ahah

References

  1. Boylan R. J., Mendelson N. H., Brooks D., Young F. E. 1972; Regulation of the bacterial cell wall: analysis of a mutant of Bacillus subtilisdefective in biosynthesis of teichoic acid. Journal of Bacteriology 110:281–290
    [Google Scholar]
  2. Burdett I. D. J. 1980; Analysis of sites of autolysis in Bacillus subtilis by electron microscopy. Journal of General Microbiology 120:35–49
    [Google Scholar]
  3. Buxton R. S. 1976; Prophage mutation causing heat inducibility of defective Bacillus subtilis bacteriophage PBSX. Journal of Virology 20:22–28
    [Google Scholar]
  4. Buxton R. S. 1978; A heat-sensitive lysis mutant of Bacillus subtilis 168 with a low activity of pyruvate carboxylase. Journal of General Microbiology 105:175–185
    [Google Scholar]
  5. Buxton R. S., Ward J. B. 1980; Heat-sensitive lysis mutants of Bacillus subtilis 168 blocked at three different stages of peptidoglycan synthesis. Journal of General Microbiology 120:283–293
    [Google Scholar]
  6. Chatterjee A. N., Young F. E. 1972; Regulation of the bacterial cell wall: isolation and characterization of peptidoglycan mutants of Staphylococcus aureus. Journal of Bacteriology 111:220–230
    [Google Scholar]
  7. Dedonder R. A., Lepesant J.-A., Lepesant-Kejz-Larova J., Blllault A., Steinmetz M., Kunst F. 1977; Construction of a kit of reference strains for rapid genetic mapping in Bacillus subtilis. Applied and Environmental Microbiology 33:989–993
    [Google Scholar]
  8. Dubnau D., Goldthwaite C., Smith I., Marmur J. 1967; Genetic mapping in Bacillus subtilis. Journal of Molecular Biology 27:163–185
    [Google Scholar]
  9. Edgar R. S., Lielausis I. 1964; Temperature-sensitive mutants of bacteriophage T4D. Their isolation and genetic characterization. Genetics 49:649–662
    [Google Scholar]
  10. Farmer J. L., Rothman F. 1965; Transformable thymine-requiring mutant of Bacillus subtilis. Journal of Bacteriology 89:262–263
    [Google Scholar]
  11. Fein J. E., Rogers H. J. 1976; Autolytic enzyme-deficient mutants of Bacillus subtilis168. Journal of Bacteriology 127:1427–1442
    [Google Scholar]
  12. Good C. M., Tipper D. J. 1972; Conditional mutants of Staphylococcus aureus defective in cell wall precursor synthesis. Journal of Bacteriology 111:231–241
    [Google Scholar]
  13. Gregory W. W., Gooder H. 1978; Inhibition of peptidoglycan biosynthesis at a postcytoplasmic reaction in a stable l-phase variant of Streptococcus faecium. Journal of Bacteriology 135:900–910
    [Google Scholar]
  14. Guerola N., Ingraham J. L., Cerdá-Olmedo E. 1971; Induction of closely linked mutations by nitrosoguanidine. Nature London: 230:122–124
    [Google Scholar]
  15. Henner D. J., Hoch J. A. 1980; The Bacillus subtilis chromosome. Microbiological Reviews 44:57–82
    [Google Scholar]
  16. Henner D. J., Hoch J. A. 1982; The genetic map of Bacillus subtilis. In The Molecular Biology of the Bacilli, 1 pp 1–33 Dubnau D. A. Edited by New York & London: Academic Press;
    [Google Scholar]
  17. Karamata D., Gross J. D. 1970; Isolation and genetic analysis of temperature-sensitive mutants of Bacillus subtilis defective in DNA synthesis. Molecular and General Genetics 108:277–287
    [Google Scholar]
  18. Kelleher R. J., Gooder H. 1973; Genetics and biochemistry of pyrimidine biosynthesis in Bacillus subtilis: linkage between mutations resulting in a requirement for uracil. Journal of Bacteriology 116:577–581
    [Google Scholar]
  19. Lugtenberg E. J. J., VanSchijndel-vanDam A. 1972a; Temperature-sensitive mutants of Escherichia coli K-12 with low activities of the l-alanine adding enzyme and the d-alanyl-d-alanine adding enzyme. Journal of Bacteriology 110:35–40
    [Google Scholar]
  20. Lugtenberg E. J. J., VanSchijndel-vanDam A. 1972b; Temperature-sensitive mutants of Escherichia coli K-12 with low activity of the diamino- pimelic acid adding enzyme. Journal of Bacteriology 110:41–46
    [Google Scholar]
  21. Lugtenberg E. J. J., VanSchijndel-vanDam A. 1973; Temperature-sensitive mutants of Escherichia coli K-12 with an impaired d-alanine :d-alanine ligase. Journal of Bacteriology 113:96–104
    [Google Scholar]
  22. Mauël C., Karamata D. 1984; Prophage induction in thermosensitive DNA mutants of Bacillus subtilis. Molecular and General Genetics 194:451–456
    [Google Scholar]
  23. Miyakawa T., Matsuzawa H., Matsuhashi M., Sugino Y. 1972; Cell wall peptidoglycan mutants of Escherichia coli K12: existence of two clusters of genes, mra and mrb, for cell wall peptidoglycan biosynthesis. Journal of Bacteriology 112:950–958
    [Google Scholar]
  24. Piggot P. J., Hoch J. A. 1985; Revised genetic linkage map of Bacillus subtilis. Microbiological Reviews 49:158–179
    [Google Scholar]
  25. Pooley H. M. 1976; Turnover and spreading of old wall during surface growth of Bacillus subtilis. Journal of Bacteriology 125:1127–1138
    [Google Scholar]
  26. Pooley H. M., Karamata D. 1983; Correlation of cell wall turnover and autolytic activity in fla and supermotile mutants of Bacillus subtilis. In The Target of Penicillin, pp 279–284 Hakenbeck R., Höltje J.-V., Labischinski H. Edited by Berlin & New York:: W. de Gruyter;
    [Google Scholar]
  27. Pooley H. M., Karamata D. 1984; Genetic analysis of autolysin-deficient and flagellaless mutants of Bacillus subtilis. Journal of Bacteriology 160:1123–1129
    [Google Scholar]
  28. Potvin B. W., Kelleher R. J., Gooder H. 1975; Pyrimidine biosynthetic pathway of Bacillus subtilis. Journal of Bacteriology 123:604–615
    [Google Scholar]
  29. Reusch V. M., Panos C. 1976; Defective synthesis of lipid intermediates for peptidoglycan formation in a stabilized l-form of Streptococcus pyogenes. Journal of Bacteriology 126:300–311
    [Google Scholar]
  30. Rogers H. J., Thurman P. F., Taylor C., Reeve J. N. 1974; Mucopeptide synthesis by rod mutants of Bacillus subtilis. Journal of General Microbiology 85:335–350
    [Google Scholar]
  31. Rogers H. J., Perkins H. R., Ward J. B. 1980 Microbial Cell Walls and Membranes London:: Chapman & Hall.;
    [Google Scholar]
  32. Siegel E. C., Marmur J. 1969; Temperature- sensitive induction of bacteriophage in Bacillus subtilis 168. Journal of Virology 4:610–618
    [Google Scholar]
  33. Tomasz A., Höltje J.-V. 1977; Murein hydrolases and the lytic and killing action of penicillin. In Microbiology - 1977, pp 209–215 Schlessinger D. Edited by Washington, DC: American Society for Microbiology;
    [Google Scholar]
  34. Venkateswaran P. S., Wu H. C. 1972; Isolation and characterization of a phosphomycin-resistant mutant of Escherichia coli K-12. Journal of Bacteriology 110:935–944
    [Google Scholar]
  35. Ward J. B. 1975; Peptidoglycan synthesis in l-phase variants of Bacillus licheniformis and Bacillus subtilis. Journal of Bacteriology 124:668–678
    [Google Scholar]
  36. Ward J. B., Zahler S. A. 1973; Genetic studies of leucine biosynthesis in Bacillus subtilis. Journal of Bacteriology 116:719–726
    [Google Scholar]
  37. Wijsman H. J. W. 1972; The characterization of an alanine racemase mutant of Escherichia coli. Genetical Research 20:269–277
    [Google Scholar]
  38. Wyrick P. B., Rogers H. J. 1973; Isolation and characterization of cell wall-defective variants of Bacillus subtilis and Bacillus licheniformis. Journal of Bacteriology 116:456–465
    [Google Scholar]
  39. Young F. E., Smith C., Reilly B. E. 1969; Chromosomal location of genes regulating resistance to bacteriophage in Bacillus subtilis. Journal of Bacteriology 98:1087–1097
    [Google Scholar]
  40. Young M. 1975; Genetic mapping of sporulation operons in Bacillus subtilis using a thermosensitive sporulation mutant. Journal of Bacteriology 122:1109–1116
    [Google Scholar]
  41. Young F. E., Wilson G. A. 1976; Revision of the linkage map of Bacillus subtilis. In Handbook of Biochemistry and Molecular Biology, 2,3rd edn,686–703 Fasman G. D. Edited by Boca Raton: CRC Press;
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-133-5-1159
Loading
/content/journal/micro/10.1099/00221287-133-5-1159
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error