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Volume 133, Issue 5

Thermosensitive Mutants Which Lyse at the Non-permissive Temperature Free

Abstract

A collection of 655 thermosensitive mutants of 168, obtained by indirect selection, was screened for those lysing at the non-permissive temperature. Thirty-three mutations thus identified were distributed by transformation into eight linkage groups designated to . The distribution was non-random. With the exception of group A, all groups were small, suggesting that mutations identified in each of them may map in one gene only. Linkage groups identified here were mapped in four different regions of the chromosome and their positions relative to reference markers were the following: (i) ; (ii) ; (iii) ; and (iv) . Kinetics of -acetyl-D-[1-C]glucosamine incorporation revealed that groups A, B, C, D and F are deficient in peptidoglycan synthesis at the restrictive temperature. In group G, anomalies at the cell wall level were suggested by incorporation and growth curves. It appears that in almost all known cases, thermosensitive lysis mutations in either affect genes involved in peptidoglycan synthesis or lead, more or less directly, to induction of prophages.

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© Society for General Microbiology 1987
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1987-05-01
2024-04-18
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Thermosensitive Bacillus Subtilis Mutants Which Lyse at the Non-permissive Temperature
Microbiology 133, 1159 (1987); https://doi.org/10.1099/00221287-133-5-1159
/content/journal/micro/10.1099/00221287-133-5-1159
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