Phage tf-1 is a filamentous phage which is about 800 nm in length, 10 nm in width and has slightly tapered ends. The phage was isolated from sewage and formed plaques or propagated only on Escherichia coli, Salmonella typhimurium and Klebsiella oxytoca strains harbouring the IncT plasmid pIN25 at 30 ° It adsorbed in large numbers to pIN25-encoded long thick flexible conjugative pili formed at 30° and also to the short form of these pili synthesized at 37 ° The reason for the failure to form plaques at 37 ° is not known. The adsorption site is a short length of the pilus shaft extending 100–200 nm back from the distal tip. Efficient phage tf-1 adsorption to the same site was found for pili determined by other IncT plasmids in spite of the fact that phage tf-1 did not plate or propagate on strains harbouring them. However, areas of specific partial clearing on lawns of these plasmid-containing bacteria were produced by phage in high concentrations. Lack of plaque-formation could be due to inefficient intracellular assembly coupled to avid adsorption of any liberated phage to pili. The phage differs from all but one other filamentous phage by being sensitive to diethyl ether.
AckermannH.-W.,
AudurierA.,
BerthiaumeL.,
JonesL. A.,
MayoJ. A.,
VidaverA. K.1978; Guidelines for bacteriophage characterization. Advances in Virus Research 23:1–24
BradleyD. E.1980b; Determination of pili by conjugative bacterial drug resistance plasmids of incompatibility groups B, C, H, J, K, M, V and X. Journal of Bacteriology 141:828–837
BradleyD. E.1984; Characteristics and function of thick and thin conjugative pili determined by transfer-derepressed plasmids of incompatibility groups I1, I2, I5, B, K. and Z. Journal of General Microbiology 130:1489–1502
BradleyD. E.,
TaylorD. E.,
CohenD. R.1980; Specification of surface mating systems among conjugative drug resistance plasmids in Escherichia coli K-12. Journal of Bacteriology 143:1466–1470
CoetzeeJ. N.1983; Lysogeny in Proteus rettgeri and the host-range of P. rettgeri and P. hauseri bacteriophages. Journal of General Microbiology 131:219–229
CoetzeeJ. N.,
LecatsasG.,
CoetzeeW. F.,
HedgesR. W.1979; Properties of R plasmid R772 and the corresponding pilus-specific phage PR772. Journal of General Microbiology 110:263–273
CoetzeeJ. N.,
SirgelF. A.,
LecatsasG.1980; Properties of a filamentous phage which adsorbs to pili coded by plasmids of the IncI complex. Journal of General Microbiology 117:547–551
CoetzeeJ. N.,
BradleyD. E.,
HedgesR. W.,
FlemingJ.,
LecatsasG.1983; Bacteriophage M: an incompatibility group M plasmid-specific phage. Journal of General Microbiology 129:2271–2276
CoetzeeJ. N.,
BradleyD. E.,
FlemingJ.,
DutoitL.,
HughesV. M.,
HedgesR. W.1985a; Phage pilHα: a phage which adsorbs to IncHI and IncHII plasmid-coded pili. Journal of General Microbiology 131:1115–1121
CoetzeeJ. N.,
BradleyD. E.,
LecatsasG.,
Du ToitL.,
HedgesR. W.1985b; Bacteriophage D: an IncD group plasmid-specific phage. Journal of General Microbiology 131:3375–3383
CoetzeeJ. N.,
BradleyD. E.,
HedgesR. W.,
HughesV. M.,
McconnellM.,
Du ToitL.,
TweehuysenM.1986; Bacteriophages F0lac h, SR, SF: phages which adsorb to pili encoded by plasmids of the new S-complex. Journal of General Microbiology 132:2907–2917
HohnB.,
Von SchützH.,
MarvinD. A.1971; Filamentous bacterial viruses. II. Killing of bacteria by abortive infection with fd. Journal of Molecular Biology 56:155–165
HuaT.-C.,
SchollD. R.,
JollickJ. D.1981; Functional modification of the plasmid RP1-specified pilus by Caulobacter vibrioides
. Journal of General Microbiology 124:119–128
JacobA. E.,
ShapiroJ. A.,
YamamotoL.,
SmithD. I.,
CohenS. N.,
BergD.1977; Plasmids studied in Escherichia coli and other enteric bacteria. In DNA Insertion Elements, Plasmids and Episomes, pp 607–638BukhariA. I.,
ShapiroJ. A.,
AdhyaS. L.
Edited by Cold Spring Harbor, New York: Cold Spring Harbor Laboratory;
KuoT. T.,
HuangT.-C.,
WuR.-Y.,
YangW.-M.1967; Characterization of three bacteriophages of Xanthomonas oryzae (Uyeda et Ishiyame) Dowson. Botanical Bulletin of Academia sinica 8:246–254
LevyS. B.,
HedgesR. W.,
SullivanF.,
MedeirosA. A.,
SosrosputroH.1985; Multiple antibiotic resistance plasmids in Enterobacteriaceae isolated from diarrhoeal specimens of hospitalized children in Indonesia. Journal of Antimicrobial Chemotherapy 16:7–16
SchwartzM.1980; Interaction of phages with their receptor proteins. In Bacterial Viruses, Receptors and Recognition, series B, vol. 6, Virus Receptors, part 1 pp 61–94RandallL.,
PhilipsonL.
Edited by London: Chapman & Hall;
ToC. M.,
ToA.,
BrintonC. C.1975; A new epiviral pilus, the E pilus, and a new RNA pilus phage pilE/R1. Abstracts of the Annual Meeting of the American Society for Microbiology p. 259 abstract S276
YokotaT.,
KanamaruY.,
MoriR.,
AkibaT.1969; Recombination between a thermosensitive kanamycin resistance factor and a non-thermosensitive multiple drug resistance factor. Journal of Bacteriology 98:863–873