1887

Microbiology

Volume 133, Issue 4

Phage tf-1: A Filamentous Bacteriophage Specific for Bacteria Harbouring the IncT Plasmid pIN25 Free

Abstract

Phage tf-1 is a filamentous phage which is about 800 nm in length, 10 nm in width and has slightly tapered ends. The phage was isolated from sewage and formed plaques or propagated only on and strains harbouring the IncT plasmid pIN25 at 30 ° It adsorbed in large numbers to pIN25-encoded long thick flexible conjugative pili formed at 30° and also to the short form of these pili synthesized at 37 ° The reason for the failure to form plaques at 37 ° is not known. The adsorption site is a short length of the pilus shaft extending 100–200 nm back from the distal tip. Efficient phage tf-1 adsorption to the same site was found for pili determined by other IncT plasmids in spite of the fact that phage tf-1 did not plate or propagate on strains harbouring them. However, areas of specific partial clearing on lawns of these plasmid-containing bacteria were produced by phage in high concentrations. Lack of plaque-formation could be due to inefficient intracellular assembly coupled to avid adsorption of any liberated phage to pili. The phage differs from all but one other filamentous phage by being sensitive to diethyl ether.

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  • Published Online:
© Society for General Microbiology 1987
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1987-04-01
2021-07-31
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References

  1. Ackermann H.-W., Audurier A., Berthiaume L., Jones L. A., Mayo J. A., Vidaver A. K. 1978; Guidelines for bacteriophage characterization. Advances in Virus Research 23:1–24
     [Google Scholar]
  2. Adams M. H. 1950; Methods of study of bacterial viruses. Methods in Medical Research 2:1–73
     [Google Scholar]
  3. Amako K., Yasunaka K. 1977; Ether induced morphological alteration of Pf-1 filamentous phage. Nature, London 267:862–863
     [Google Scholar]
  4. Beumer J., Hannecart-Pokorni E., Godard C. 1984; Bacteriophage receptors. Bulletin de l’Institut Pasteur 82:173–253
     [Google Scholar]
  5. Bradley D. E. 1977; Characterization of pili determined by drug resistance plasmids R71lb and R778b. Journal of General Microbiology 102:349–363
     [Google Scholar]
  6. Bradley D. E. 1980a; Morphological and serological relationships of conjugative pili. Plasmid 4:155–169
     [Google Scholar]
  7. Bradley D. E. 1980b; Determination of pili by conjugative bacterial drug resistance plasmids of incompatibility groups B, C, H, J, K, M, V and X. Journal of Bacteriology 141:828–837
     [Google Scholar]
  8. Bradley D. E. 1984; Characteristics and function of thick and thin conjugative pili determined by transfer-derepressed plasmids of incompatibility groups I1, I2, I5, B, K. and Z. Journal of General Microbiology 130:1489–1502
     [Google Scholar]
  9. Bradley D. E. 1985; Transfer systems of K88 and K99 plasmids. Plasmid 13:118–128
     [Google Scholar]
  10. Bradley D. E., Whelan J. 1985; Conjugation systems of IncT plasmids. Journal of General Microbiology 131:2665–2671
     [Google Scholar]
  11. Bradley D. E., Taylor D. E., Cohen D. R. 1980; Specification of surface mating systems among conjugative drug resistance plasmids in Escherichia coli K-12. Journal of Bacteriology 143:1466–1470
     [Google Scholar]
  12. Bradley D. E., Coetzee J. N., Bothma T., Hedges R. W. 1981a; Phage X: a plasmid- dependent, broad host range, filamentous bacterial virus. Journal of General Microbiology 126:389–396
     [Google Scholar]
  13. Bradley D. E., Coetzee J. N., Bothma T., Hedges R. W. 1981b; Phage t: a group T plasmid- dependent bacteriophage. Journal of General Micro-
     [Google Scholar]
  14. Bradley D. E., Coetzee J. N., Hedges R. W. 1983; Incl2 plasmids specify sensitivity to filamentous bacteriophage IKe. Journal of Bacteriology 154:505–507
     [Google Scholar]
  15. Coetzee J. N. 1972; Genetics of the proteus group. Annual Review of Microbiology 26:23–54
     [Google Scholar]
  16. Coetzee J. N. 1983; Lysogeny in Proteus rettgeri and the host-range of P. rettgeri and P. hauseri bacteriophages. Journal of General Microbiology 131:219–229
     [Google Scholar]
  17. Coetzee J. N., Datta N., Hedges R. W. 1972; R factors from Proteus rettgeri . Journal of General Microbiology 72:543–552
     [Google Scholar]
  18. Coetzee J. N., Lecatsas G., Coetzee W. F., Hedges R. W. 1979; Properties of R plasmid R772 and the corresponding pilus-specific phage PR772. Journal of General Microbiology 110:263–273
     [Google Scholar]
  19. Coetzee J. N., Sirgel F. A., Lecatsas G. 1980; Properties of a filamentous phage which adsorbs to pili coded by plasmids of the IncI complex. Journal of General Microbiology 117:547–551
     [Google Scholar]
  20. Coetzee J. N., Bradley D. E., Hedges R. W. 1982; Phages lα and I2-2: IncI plasmid-dependent bacteriophages. Journal of General Microbiology 128:2797–2804
     [Google Scholar]
  21. Coetzee J. N., Bradley D. E., Hedges R. W., Fleming J., Lecatsas G. 1983; Bacteriophage M: an incompatibility group M plasmid-specific phage. Journal of General Microbiology 129:2271–2276
     [Google Scholar]
  22. Coetzee J. N., Bradley D. E., Fleming J., Dutoit L., Hughes V. M., Hedges R. W. 1985a; Phage pilHα: a phage which adsorbs to IncHI and IncHII plasmid-coded pili. Journal of General Microbiology 131:1115–1121
     [Google Scholar]
  23. Coetzee J. N., Bradley D. E., Lecatsas G., Du Toit L., Hedges R. W. 1985b; Bacteriophage D: an IncD group plasmid-specific phage. Journal of General Microbiology 131:3375–3383
     [Google Scholar]
  24. Coetzee J. N., Bradley D. E., Hedges R. W., Hughes V. M., Mcconnell M., Du Toit L., Tweehuysen M. 1986; Bacteriophages F0 lac h, SR, SF: phages which adsorb to pili encoded by plasmids of the new S-complex. Journal of General Microbiology 132:2907–2917
     [Google Scholar]
  25. Hohn B., Von Schütz H., Marvin D. A. 1971; Filamentous bacterial viruses. II. Killing of bacteria by abortive infection with fd. Journal of Molecular Biology 56:155–165
     [Google Scholar]
  26. Hua T.-C., Scholl D. R., Jollick J. D. 1981; Functional modification of the plasmid RP1-specified pilus by Caulobacter vibrioides . Journal of General Microbiology 124:119–128
     [Google Scholar]
  27. Ishaq M., Kaji A. 1980; Mechanism of T4phage restriction by plasmid RTS 1. Journal of Biological Chemistry 255:4040–4047
     [Google Scholar]
  28. Jacob A. E., Shapiro J. A., Yamamoto L., Smith D. I., Cohen S. N., Berg D. 1977; Plasmids studied in Escherichia coli and other enteric bacteria. In DNA Insertion Elements, Plasmids and Episomes, pp 607–638 Bukhari A. I., Shapiro J. A., Adhya S. L. Edited by Cold Spring Harbor, New York: Cold Spring Harbor Laboratory;
     [Google Scholar]
  29. Khatoon H., Iyer R. V., Iyer V. N. 1972; A new filamentous bacteriophage with sex-factor specificity. Virology 48:145–155
     [Google Scholar]
  30. Kuo T. T., Huang T.-C., Wu R.-Y., Yang W.-M. 1967; Characterization of three bacteriophages of Xanthomonas oryzae (Uyeda et Ishiyame) Dowson. Botanical Bulletin of Academia sinica 8:246–254
     [Google Scholar]
  31. Levy S. B., Hedges R. W., Sullivan F., Medeiros A. A., Sosrosputro H. 1985; Multiple antibiotic resistance plasmids in Enterobacteriaceae isolated from diarrhoeal specimens of hospitalized children in Indonesia. Journal of Antimicrobial Chemotherapy 16:7–16
     [Google Scholar]
  32. Marvin D. A., Hoffmann-Berling H. 1963; Physical and chemical properties of two new small bacteriophages. Nature, London 197:517–518
     [Google Scholar]
  33. Marvin D. A., Hohn B. 1969; Filamentous bacterial viruses. Bacteriological Reviews 33:172–209
     [Google Scholar]
  34. Matsumoto H., Tazaki T., Hosogaya S. 1973; A generalized transducing phage of Serratia marces- cens . Japanese Journal of Microbiology 17:473–479
     [Google Scholar]
  35. Matthew M., Hedges R. W. 1976; Analytical isoelectric focussing of R-factor determined β- lactamases: correlation with plasmid compatibility. Journal of Bacteriology 125:713–718
     [Google Scholar]
  36. Meynell G. G., Lawn A. M. 1968; Filamentous phages specific of the I sex factor. Nature, London 217:1184–1186
     [Google Scholar]
  37. Minamishima Y., Takeya K., Ohnishi Y., Amako K. 1968; Physicochemical and biological properties of fibrous Pseudomonas bacteriophages. Journal of Virology 2:208–213
     [Google Scholar]
  38. Odakura Y., Hashimoto H., Mitsuhashi S. 1977; Temperature sensitive R plasmids isolated from Proteus strains. Microbiology and Immunology 21:621–629
     [Google Scholar]
  39. Salivar W. O., Tzagoloff H., Pratt D. 1964; Some physical-chemical and biological properties of the rod-shaped coliphage M13. Virology 24:359–371
     [Google Scholar]
  40. Schwartz M. 1980; Interaction of phages with their receptor proteins. In Bacterial Viruses, Receptors and Recognition, series B, vol. 6, Virus Receptors, part 1 pp 61–94 Randall L., Philipson L. Edited by London: Chapman & Hall;
     [Google Scholar]
  41. Slrgel F. A., Coetzee J. N., Hedges R. W., Lecatsas G. 1981; Phage C-l: an IncC group plasmid-specific phage. Journal of General Microbiology 122:155–160
     [Google Scholar]
  42. Spratt B. G., Rowbury R. H., Meynell G. G. 1973; The plasmid of Salmonella typhimurium LT2. Molecular and General Genetics 121:347–353
     [Google Scholar]
  43. Terawaki Y., Rownd R. 1972; Replication of the R factor Rtsl in Proteus mirabilis . Journal of Bacteriology 109:492–498
     [Google Scholar]
  44. Terawakie Y., Takayasu H., Akiba T. 1967; Thermosensitive replication of a kanamycin resistance factor. Journal of Bacteriology 94:687–690
     [Google Scholar]
  45. To C. M., To A., Brinton C. C. 1975; A new epiviral pilus, the E pilus, and a new RNA pilus phage pilE/R1. Abstracts of the Annual Meeting of the American Society for Microbiology p. 259 abstract S276
    [Google Scholar]
  46. Watanabe M., August J. T. 1967; Methods for selecting RNA bacteriophage. Methods in Virology 3:337–350
     [Google Scholar]
  47. Yokota T., Kanamaru Y., Mori R., Akiba T. 1969; Recombination between a thermosensitive kanamycin resistance factor and a non-thermosensitive multiple drug resistance factor. Journal of Bacteriology 98:863–873
     [Google Scholar]
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Phage tf-1: A Filamentous Bacteriophage Specific for Bacteria Harbouring the IncT Plasmid pIN25
Microbiology 133, 953 (1987); https://doi.org/10.1099/00221287-133-4-953
/content/journal/micro/10.1099/00221287-133-4-953
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