SUMMARY: The gene encoding a glucosyltransferase which synthesized water-insoluble glucan, , previously cloned from strain MFe28 (mutans serotype h) into a bacteriophage λ vector, was subcloned into the plasmid pBR322. The recombinant plasmid was stable in and was efficiently expressed. The GTF-I expressed in was compared to the corresponding enzymes in strains MFe28 (serotype ), B13 (serotype ) and 6715 (serotype ) and shown to resemble them closely in molecular mass and isoelectric point. The insoluble glucan produced by GTF-I from recombinant consisted of 1,3-α-d-glycosyl residues (~90%). An internal fragment of the gene was used as a probe in hybridization experiments to demonstrate the presence of homologous sequences in chromosomal DNA of other streptococci of the mutans group.


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