1887

Abstract

At least two aminoglycoside-resistance determinants from have been cloned separately in . In each case, resistance (to kanamycin plus apramycin or to kanamycin plus gentamicin) was expressed at the level of the ribosome and involved specific methylation of 16S ribosomal RNA. Hybridization and restriction analysis revealed that related genes were present in other aminoglycoside-producing actinomycetes.

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/content/journal/micro/10.1099/00221287-133-4-915
1987-04-01
2024-04-25
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References

  1. Benveniste R., Davies J. 1973; Aminoglycoside antibiotic-inactivating enzymes in actinomycetes similar to those present in clinical isolates of antibiotic-resistance bacteria. Proceedings of the National Academy of Sciences of the United States of America 70:2276–2280
    [Google Scholar]
  2. Chater K. F., Hopwood D. A., Kieser T., Thompson C. J. 1982; Gene cloning in Strepto- myces. . Current Topics in Microbiology and Immunology 96:69–95
    [Google Scholar]
  3. Crameri R., Davies J. E. 1986; Increased production of aminoglycosides associated with amplified antibiotic resistance genes. Journal of Antibiotics 39:128–135
    [Google Scholar]
  4. Davies J. 1980; Enzymes modifying aminocyclitol antibiotics and their roles in resistance determination and biosynthesis. In Aminocyclitol Antibiotics pp. 323–334 Rinehart K. L. JR Sumai T. Edited by Washington, DC:: American Chemical Society;
    [Google Scholar]
  5. Feinberg A. P., Vogelstein B. 1984; A technique for radiolabelling DNA restriction endonuclease fragments to high specific activity. Analytical Biochemistry 137:266–267
    [Google Scholar]
  6. Hopwood D. A., Bibb M. J., Chater K. F., Janssen G. R., Malpartida F., Smith C. P. 1986; Regulation of gene expression in antibiotic-producing Streptomyces. Symposia of the Society for General Microbiology 39:251–276
    [Google Scholar]
  7. Katz E., Thompson C. J., Hopwood D. A. 1983; Cloning and expression of the tyrosinase gene from Streptomyces antibioticus in Streptomyces lividans. Journal of General Microbiology 129:2703–2714
    [Google Scholar]
  8. Piendl W., Böck A., Cundliffe E. 1984; Involvement of 16s ribosomal RNA in resistance of the aminoglycoside-producers Streptomyces tenjimariensis. Streptomyces tenebrarius and Micromonospora purpurea. Molecular and General Genetics 197:24–29
    [Google Scholar]
  9. Skeggs P. A., Thompson J., Cundliffe E. 1985; Methylation of 16S ribosomal RNA and resistance to aminoglycoside antibiotics in clones of Streptomyces lividans carrying DNA from Streptomyces tenjimariensis. Molecular and General Genetics 200:415–421
    [Google Scholar]
  10. Thompson C. J., Skinner R. H., Thompson J., Ward J. M., Hopwood D. A., Cundliffe E. 1982 a; Biochemical characterization of resistance determinants cloned from antibiotic-producing streptomycetes. Journal of Bacteriology 151:678–685
    [Google Scholar]
  11. Thompson C. J., Ward J. M., Hopwood D. A. 1982b; Cloning of antibiotic resistance and nutritional genes in streptomycetes. Journal of Bacteriology 151:668–677
    [Google Scholar]
  12. Thompson J., Skeggs P. A., Cundliffe E. 1985; Methylation of 16S ribosomal RNA and resistance to the aminoglycoside antibiotics gentamicin and kanamycin determined by DNA from the gentamicin producer, Micromonospora purpurea. Molecular and General Genetics 201:168–173
    [Google Scholar]
  13. Yamamoto H., Hotta K., Okami Y., Umezawa H. 1982; Mechanism of resistance to aminoglycoside antibiotics in nebramycin-producing Streptomyces tenebrarius. Journal of Antibiotics 35:1020–1025
    [Google Scholar]
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