RT Journal Article SR Electronic(1) A1 Terpstra, W. J. A1 Schoone, G. J. A1 Ligthart, G. S. A1 Ter Schegget, J.YR 1987 T1 Detection of Leptospira Interrogans in Clinical Specimens by in situ Hybridization Using Biotin-Labelled DNA Probes JF Microbiology, VO 133 IS 4 SP 911 OP 914 DO https://doi.org/10.1099/00221287-133-4-911 PB Microbiology Society, SN 1465-2080, AB In situ DNA hybridization using biotin-labelled leptospiral DNA was performed on clinical specimens to investigate its usefulness as a technique for the identification of Leptospira interrogans. The applicability of this test in blood, urine and liver smears was demonstrated. In situ DNA hybridization can be completed in only 4 h and it combines the advantage of visualization of the leptospiral morphology with the specificity of the hybridization reaction. No cross-hybridization was observed with other bacteria. This study shows that hybridization in situ can be simple to perform and may contribute to a rapid diagnosis., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-133-4-911