SUMMARY: DNA hybridization using biotin-labelled leptospiral DNA was performed on clinical specimens to investigate its usefulness as a technique for the identification of . The applicability of this test in blood, urine and liver smears was demonstrated. DNA hybridization can be completed in only 4 h and it combines the advantage of visualization of the leptospiral morphology with the specificity of the hybridization reaction. No cross-hybridization was observed with other bacteria. This study shows that hybridization can be simple to perform and may contribute to a rapid diagnosis.


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