@article{mbs:/content/journal/micro/10.1099/00221287-133-4-883, author = "Melasniemi, Hannes", title = "Effect of Carbon Source on Production of Thermostable α-Amylase, Pullulanase and α-Glucosidase by Clostridium Thermohydrosulfuricum", journal= "Microbiology", year = "1987", volume = "133", number = "4", pages = "883-890", doi = "https://doi.org/10.1099/00221287-133-4-883", url = "https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-133-4-883", publisher = "Microbiology Society", issn = "1465-2080", type = "Journal Article", abstract = "The anaerobic thermophile Clostridium thermohydrosulfuricum produced thermostable α-amylase, pullulanase and α-glucosidase activities during growth on starch, pullulan, dextrin or maltose. Synthesis of α-amylase and pullulanase was partially repressed by glucose, whereas α-glucosidase synthesis was not. Fructose completely repressed the synthesis of α-amylase and pullulanase but only partially that of α-glucosidase. α-Amylase and pullulanase activities were predominantly located extracellularly. However, during growth on low amounts of soluble starches (2%, w/v) virtually all activity was cell-associated. Under most conditions examined 75% or more of the α-glucosidase activity was cell-bound. The combined action of these activities produced glucose as the final end-product from amylose and pullulan digestions.", }