%0 Journal Article %A Van Schie, B. J. %A De Mooy, O. H. %A Linton, J. D. %A Van Dijken, J. P. %A Kuenen, J. G. %T PQQ-Dependent Production of Gluconic Acid by Acinetobacter, Agrobacterium and Rhizobium Species %D 1987 %J Microbiology, %V 133 %N 4 %P 867-875 %@ 1465-2080 %R https://doi.org/10.1099/00221287-133-4-867 %I Microbiology Society, %X SUMMARY: Acinetobacter Iwoffi, Azotobacter vinelandii, Agrobacterium and Rhizobium species contain quinoprotein glucose dehydrogenase apoenzyme (EC 1.1.99.17). Addition to whole cells of pyrrolo-quinoline quinone (PQQ), the prosthetic group of this enzyme, resulted in the production of gluconic acid from glucose. The in vivo reconstitution of apo-glucose dehydrogenase with PQQ was dependent on the presence of Ca2+ or Mg2+. Optimal conditions for reconstitution allowed maximal glucose dehydrogenase activity in the presence of 1–10 nmol PQQ 1−1. Synthesis of the apoenzyme of glucose dehydrogenase was not dependent on glucose in the growth media. The physiological significance of the synthesis of apo-glucose dehydrogenase, as found in a variety of bacteria, is discussed. %U https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-133-4-867