1887

Abstract

SUMMARY: and species contain quinoprotein glucose dehydrogenase apoenzyme (EC 1.1.99.17). Addition to whole cells of pyrrolo-quinoline quinone (PQQ), the prosthetic group of this enzyme, resulted in the production of gluconic acid from glucose. The reconstitution of apo-glucose dehydrogenase with PQQ was dependent on the presence of Ca or Mg. Optimal conditions for reconstitution allowed maximal glucose dehydrogenase activity in the presence of 1–10 nmol PQQ 1. Synthesis of the apoenzyme of glucose dehydrogenase was not dependent on glucose in the growth media. The physiological significance of the synthesis of apo-glucose dehydrogenase, as found in a variety of bacteria, is discussed.

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1987-04-01
2021-10-16
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