SUMMARY: and species contain quinoprotein glucose dehydrogenase apoenzyme (EC Addition to whole cells of pyrrolo-quinoline quinone (PQQ), the prosthetic group of this enzyme, resulted in the production of gluconic acid from glucose. The reconstitution of apo-glucose dehydrogenase with PQQ was dependent on the presence of Ca or Mg. Optimal conditions for reconstitution allowed maximal glucose dehydrogenase activity in the presence of 1-10 nmol PQQ 1. Synthesis of the apoenzyme of glucose dehydrogenase was not dependent on glucose in the growth media. The physiological significance of the synthesis of apo-glucose dehydrogenase, as found in a variety of bacteria, is discussed.


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