1887

Abstract

SUMMARY: The effect of Cd poisoning of on Ca, Cd and [C]tetraphenyl phosphonium (TPP) uptake and cell pH was examined. At Cd concentrations that produced substantial K+ efflux the rates of uptake of Ca, Cd and [C]TPP increased progressively during incubation of the cells with Cd, and the cell pH was lowered concomitantly. The initial rates of uptake of the divalent cations and of TPP were increased in cells pre-loaded with Cd, which shows that stimulation of the ion fluxes was exerted by the Cd that accumulated in the cells. The distribution ratio of TPP between cells and medium, however, was decreased by Cd. Although hyperpolarization of the cell membrane by Cd cannot be excluded, it is argued that Cd primarily stimulated divalent cation uptake by increasing the cation permeability of the cell membrane allowing the cations to enter the cells more easily.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-133-4-843
1987-04-01
2021-07-27
Loading full text...

Full text loading...

/deliver/fulltext/micro/133/4/mic-133-4-843.html?itemId=/content/journal/micro/10.1099/00221287-133-4-843&mimeType=html&fmt=ahah

References

  1. Ahlers J., Rösick E. 1985; Cd2+-uptake and influence on the kinetics of yeast plasma membrane ATPase. In Mechanistic and Energetic Aspects of Membrane Transport in Yeast pp 21–22 Borst-Pauwels G. W. F. H., Theuvenet A. P. R. Edited by Roermond: Arts drukkerij b.v.;
    [Google Scholar]
  2. Bitton G., Koopman B., Wang H. 1984; Baker’s yeast assay procedure for testing heavy metal toxicity. Bulletin of Environmental Contamination and Toxicology 32:80–84
    [Google Scholar]
  3. Borst-Pauwels G.W.F.H., Dobbelmann J. 1972; Determination of the yeast cell pH. Actabotanicaneerlandica 21:149–154
    [Google Scholar]
  4. Borst-Pauwels G.W.F.H., Theuvenet A. P. R., Stols A. L. H. 1983; All-or-none interactions of inhibitors of the plasma membrane ATPase of Saccharomyces cerevisiae. . Biochimica et biophysicaacta 732:186–192
    [Google Scholar]
  5. Borst-Pauwels G.W.F.H., Boxman A. W., Theuvenet A. P. R. 1984; Energetisation and regulation of Rb+ (K+) transport in yeast. In Environmental Regulation of Microbial Metabolism pp 369–376 Kualev I. S., Dawes E. A., Tempest D. W. Edited by New York: Academic Press;
    [Google Scholar]
  6. Borst-Pauwels G.W.F.H., Theuvenet A. P. R., Boxman A. W., Peters P. H. J., Dobbelmann J. 1986; Interaction of calmodulin antagonists with yeast. Comparison with the effect of miconazole, Dio-9 and ethidium upon Sr2+ uptake, K+ efflux and plasmamembrane ATPase activity. Journal of Bioelectrochemistry and Bioenergetics 16:519–530
    [Google Scholar]
  7. Boxman A. W., Barts P.W.J.A., Borst-Pauwels G.W.F.H. 1982; Some characteristics of tetra- phenylphosphonium uptake into Saccharomyces cerevisiae. . Biochimica et biophysicaacta 686:13–18
    [Google Scholar]
  8. Dufour J. P., Boutry M., Goffeau A. 1980; Plasmamembrane ATPase of yeast. Comparative inhibition studies on the purified and membrane bound enzymes. Journal of Biological Chemistry 225:5735–5741
    [Google Scholar]
  9. Eilam Y. 1983; Membrane effects of phenothiazines in yeasts. I. Stimulation of calcium and potassium fluxes. Biochimica et biophysicaacta 733:242–248
    [Google Scholar]
  10. Eilam Y. 1984; Effects of phenothiazines on inhibition of plasma membrane ATPase and hyperpolarization of cell membranes in the yeast Saccharomyces cerevisiae. . Biochimicaetbiophysicaacta 769:601–610
    [Google Scholar]
  11. Eilam Y., Lavi H., Grossowicz N. 1985; Cytoplasmic Ca2+ homeostasis maintained by a vacuolar Ca2+ transport system in the yeast Saccharomyces cerevisiae. . Journal of General Microbiology 131:2555–2564
    [Google Scholar]
  12. Foury F., Boutry M., Goffeau A. 1977; Efflux of potassium induced by Dio-9, a plasma membrane ATPase inhibitor in the yeast Schizosaccharomycespombe. . Journal of Biological Chemistry 252:4577–4583
    [Google Scholar]
  13. Gadd G. M., Mowll J. L. 1983; The relationship between cadmium uptake, potassium release and viability in Saccharomyces cerevisiae. . FEMS Microbiology Letters 16:45–48
    [Google Scholar]
  14. Heldwein R., Tromballa H. W., Broda E. 1977; Aufnahme von Cobalt, Blei und Cadmium durchBackerhefe. Zeitschrift für AlgemeineMikro- biologie 17:299–308
    [Google Scholar]
  15. Kessels B. G. F., Belde P. J. M., Borst-Pauwels G.W.F.H. 1985; Protection of Saccharomyces cerevisiae against Cd2+ toxicity by Ca2+ . Journal of General Microbiology 131:2533–2537
    [Google Scholar]
  16. Macara I. G. 1978; Accommodation of yeast to toxic levels of cadmium ions. Journal of General Microbiology 104:321–324
    [Google Scholar]
  17. Norris P. R., Kelly K. P. 1977; Accumulation of cadmium and cobalt by Saccharomyces cerevisiae. . Journal of General Microbiology 99:317–324
    [Google Scholar]
  18. Peña A. 1978; Effect of ethidiiim bromide on Ca2+ uptake by yeast. Journal of Membrane Biology 42:199–213
    [Google Scholar]
  19. Peña A., Ramirez G. 1976; Interaction of ethidium bromide with the transport system for monovalent cations in yeast. Journal of Membrane Biology 22:369–384
    [Google Scholar]
  20. Roomans G. M., Theuvenet A. P. R., Van Den Berg T. P. R., Borst-Pauwels G.W.F.H. 1979; Kinetics of Ca2+ and Sr2+ uptake by yeast.Effects of pH, cations and phosphate. Biochimica et biophysicaacta 551:187–196
    [Google Scholar]
  21. Serrano R. 1980; Effect of ATPase inhibitors on the proton pump of respiratory-deficient yeast. European Journal of Biochemistry 105:419–424
    [Google Scholar]
  22. Theuvenet A. P. R., Van De Wijngaard W. M. H., Vanc De Rijke J. W., Borst-Pauwels G.W.F.H. 1984; Application of 9-aminoacridine as a probe of the surface potential experienced by cation transporters in the plasma membrane of yeast cells. Biochimica et biophysicaacta 775:161–168
    [Google Scholar]
  23. Theuvenet A. P. R., Nieuwenhuis G.J.W.M., Van De Mortel J., Borst-Pauwels G.W.F.H. 1986; Effect of ethidium bromide and DEAE- dextran on divalent cation accumulation in yeast.Evidence for an ion-selective extrusion pump for divalent cations. Biochimica et biophysicaacta 855:383–390
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-133-4-843
Loading
/content/journal/micro/10.1099/00221287-133-4-843
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error