1887

Abstract

SUMMARY: The chemical composition and immunobiological activities and of sodium dodecyl sulphate extracts (SDS-SE) derived from periodontopathic bacteria (three strains of , two strains of , and one strain of ) were investigated. The main components of SDS-SE were protein and lipid, with negligible amounts of peptidoglycan and lipopolysaccharide. Immunopotentiating activity was detected in both delayed-type hypersensitivity and antibody formation against the elicitation of a protein antigen with the SDS-SE preparations of ATCC 29524 and 381 and 1021. On the other hand the SDS-SE of ATCC 29522 enhanced only the induction of a delayed-type hypersensitivity response. All the SDS-SE preparations had mitogenic activity to splenocytes from BALB/c , C3H/HeN and C3H/HeJ mice. Migration-stimulating activity for human peripheral blood monocytes was detected especially in the SDS-SE preparations of ATCC 29524 and Y4. All of the SDS-SE samples inhibited [H]thymidine uptake in human gingival fibroblasts and caused degradation of the cells. The results suggest that the cell membrane components extractable with sodium dodecyl sulphate from periodontopathic bacteria are involved in the pathogenesis of periodontal disease.

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1987-04-01
2021-07-28
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References

  1. Ashwell G. 1957; Colorimetric analysis of sugars. Methods in Enzymology 3:84–90
    [Google Scholar]
  2. Baehni P., Tsai C.-C., Mcarthur W. P., Hammond B. F., Taichman N. S. 1979; Interaction of inflammatory cells and oral microorganisms. VIII. Detection of leukotoxic activity of a plaque-derived gram-negative microorganism. Infection and Immunity 24:233–243
    [Google Scholar]
  3. Baehni P. C., Tsai C.-C., Mcarthur W. P., Hammond B. F., Shenker B. J., Taichman N. S. 1981; Leukotoxic activity in different strains of the bacterium Actinobacillus actinomycetemcomitans isolated from juvenile periodontitis in man. Archives of Oral Biology 26:671–676
    [Google Scholar]
  4. Calhoon D. A., Mayberry W. R., Slots J. 1981; Cellular fatty acid and soluble protein composition of Actinobacillus actinomycetemcomitans and related organisms. Journal of Clinical Microbiology 14:376–382
    [Google Scholar]
  5. Dische Z., Shettles L. B. 1948; A specific color reaction of methylpentoses and a spectrophoto-metric micromethod for their determination. Journal of Biological Chemistry 175:595–603
    [Google Scholar]
  6. Filip C., Fletcher G., Wulff J. L., Earhart C. F. 1973; Solubilization of the cytroplasmic membrane of Escherichia coli by the ionic detergent sodium lauryl sarcosinate. Journal of Bacteriology 115:717–722
    [Google Scholar]
  7. Hausmann E., Luderitz O., Knox K., Weinfeld N. 1975; Structural requirements for bone resorption by endotoxin and lipoteichoic acid. Journal of Dental Research(Special Issue) B54:B94–B99
    [Google Scholar]
  8. Hayashi K. 1975; A rapid determination of sodium dodecyl sulfate with methylene blue. Analytical Biochemistry 67:503–506
    [Google Scholar]
  9. Iino Y., Hopps R. M. 1984; The bone-resorbing activities in tissue culture of lipopolysaccharides from the bacteria Actinobacillus actinomycetemcomitans, Bacteroides gingivalis and Capnocytophagaochracea isolated from human mouths. Archives of Oral Biology 29:59–63
    [Google Scholar]
  10. Ikemoto S., Katoh K., Komagata K. 1978; Cellular fatty acid composition in methanol-utilizing bacteria. Journal of General and Applied Microbiology 24:41–49
    [Google Scholar]
  11. Iwanaga S., Morita T., Harada T., Nakamura S., Niwa M., Takada K., Kimura T., Sakakibara S. 1978; Chromogenic substrates for horseshoe crab clotting enzyme. Its application for the assay of bacterial endotoxins. Haemostasis 7:183–188
    [Google Scholar]
  12. Kamen P. R. 1983; Inhibition of keratinocyte proliferation by extracts of Actinobacillus actinomycetemcomitans. Infection and Immunity 42:1191–1194
    [Google Scholar]
  13. Kiley P., Holt S. C. 1980; Characterization of the lipopolysaccharide from Actinobacillus actinomycetemcomitans Y4 and N27. Infection and Immunity 30:862–873
    [Google Scholar]
  14. Koga T., Nishihara T., Fujiwara T., Nishizawa T., Okahashi N., Noguchi T., Hamada S. 1985; Biochemical and immunobiological properties of lipopolysaccharide (LPS) from Bacteroides gingivalis and comparison with LPS from Escherichia coli. Infection and Immunity 47:638–647
    [Google Scholar]
  15. Kotani S., Narita T., Stewart-Tull D. E. S., Shimono T., Watanabe Y., Kato K., Iwata S. 1975; Immunoadjuvant activities of cell walls and their water-soluble fractions prepared from various gram-positive bacteria. Biken Journal 18:77–92
    [Google Scholar]
  16. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature; London: 227680–685
    [Google Scholar]
  17. Lambert M., Neish A. C. 1950; Rapid method for estimation of glycerol in fermentation solutions. Canadian Journal of Research 28: section B 83–89
    [Google Scholar]
  18. Lehner T. 1975; Immunological aspects of dental caries and periodontal disease. British Medical Bulletin 31:125–130
    [Google Scholar]
  19. Lowry O. H., Roberts N. R., Leiner K. Y., Wu M.-L., Farr A. L. 1954; The quantitative histochemistry of brain. 1. Chemical methods. Journal of Biological Chemistry 207:1–17
    [Google Scholar]
  20. Macrina F. L., Ranney R. R. 1982; Periodontal disease: microbiological and epidemiological aspects. In Dental Microbiology pp. 732–752 Mcghee J. P., Michalek S. M., Cassell G. H. Edited by Philadelphia: Harper & Row;
    [Google Scholar]
  21. Mayberry W. R. 1980; Hydroxy fatty acids in Bacteroides species: D-( — )-3-hydroxy-15-methylhex-adecanoate and its homologs. Journal of Bacteriology 143:582–587
    [Google Scholar]
  22. Murayama Y., Muranishi K., Okada H., Kato K., Kotani S., Takada H., Tsujimoto M., Kawasaki A., Ogawa T. 1982; Immunological activities of Capnocytophaga cellular components. Infection and Immunity 36:876–884
    [Google Scholar]
  23. Nagai A., Kurihara H., Kinoshita M., Matsuo M., Shimuzu H., Toda Y., Nomura Y., Murayama Y., Kokeguchi S., Kato K. 1984; Examination of enzyme-linked immunosorbent assay for serum antibodies against periodontally associated microorganisms. Journal of the Japanese Association of Periodontology 26:317–328 (in Japanese)
    [Google Scholar]
  24. Ogawa T., Kotani S., Fukuda K., Tsukamoto Y., Mori M., Kusumoto S., Shiba T. 1982; Stimulation of migration of human monocytes by bacterial cell walls and muramyl peptides. Infection and Immunity 38:817–824
    [Google Scholar]
  25. Ohta H., Hattori T. 1983; Agromonas oligotrophica gen. nov., sp. nov., a nitrogen-fixing oligotrophic bacterium. Antonie van Leeuwenhoek 49:429–446
    [Google Scholar]
  26. Page R. C., Schroeder H. E. 1981; Current status of the host response in chronic marginal periodontitis. Journal of Periodontology 52:477–491
    [Google Scholar]
  27. Progulske A., Mishell R., Trummel C., Holt S. C. 1984; Biological activities of Eikenella corrodens outer membrane and lipopolysaccharide. Infection and Immunity 43:178–182
    [Google Scholar]
  28. Singer R. E., Buckner B. A. 1981; Butyrate and propionate: important components of toxic dental plaque extracts. Infection and Immunity 32:458–463
    [Google Scholar]
  29. Slots J., Genco R. J. 1984; Black-pigmented Bacteroides species, Capnocytophaga species, and Actinobacillus actinomycetemcomitans in human periodontal disease: virulence factors in colonization, survival, and tissue destruction. Journal of Dental Research 63:412–421
    [Google Scholar]
  30. Stevens R. H., Sella M. N., Shapira J., Hammond B. F. 1980; Detection of a fibroblast proliferation inhibitory factor from Capnocytophaga sputigena. Infection and Immunity 27:271–275
    [Google Scholar]
  31. Stevens R. H., Gatewood C., Hammond B. F. 1983; Cytotoxicity of the bacterium Actinobacillus actinomycetemcomitans extracts in human gingival fibroblasts. Archives of Oral Biology 28:981–987
    [Google Scholar]
  32. Sveen K. 1977; The capacity of lipopolysaccharides from bacteroides, fusobacterium, and veillonella to produce skin inflammation and the local generalized Schwartzman reaction in rabbits. Journal of Periodontal Research 12:340–350
    [Google Scholar]
  33. Takada H., Tsujimoto M., Kotani S., Kusumoto S., Inage M., Shiba T., Nagao S., Yano I., Kawata S., Yokogawa K. 1979; Mitogenic effects of bacterial cell walls, their fragments, and related synthetic compounds on thymocytes and splenocytes of guinea pigs. Infection and Immunity 25:645–652
    [Google Scholar]
  34. Weissbach A., Hurwitz J. 1959; The formation of 2-keto-3-deoxyheptonic acid in extracts of Escherichia coli B. Journal Biological Chemistry 234:705–709
    [Google Scholar]
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