1887

Abstract

Zn uptake by was biphasic. The first phase was independent of metabolic energy, consisting of adsorption to the cell surface, and followed a Freundlich isotherm. The second phase was dependent on metabolic energy, ATPase activity and the transmembrane proton gradient, and consisted of uptake into the cell. Energy-dependent uptake showed Michaelis-Menten kinetics with a of 3·7μ-Zn and a of 1·6 nmol min per 10 cells at Zn concentrations below 80 μm but deviated at higher concentrations. K and Mg inhibited energy-dependent Zn uptake while Na and Ca did not. The effect of heavy metals was complex and included both inhibition and stimulation of Zn uptake. K efflux accompanied Zn uptake at all Zn concentrations but there was no simple stoichiometric relationship between the two. Toxic effects of Zn such as inhibition of H efflux and K uptake and reduction of viability were observed at all Zn concentrations and toxicity appeared to be a major factor in K efflux. Toxicity also affected the kinetics of Zn uptake, being a major cause of deviation from Michaelis-Menten kinetics. Zn was compartmented within the cell: 56 % of the total intracellular pool was in the soluble vacuolar fraction, 39 % was bound to insoluble components and only 5 % was found in the cytosol. Isolated yeast vacuoles possessed an ATP-dependent Zn uptake system whose properties were consistent with a Zn/H antiport.

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1987-03-01
2024-04-24
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References

  1. Boller T., Durr M., Wiemken A. 1975; Characterization of a specific transport system for arginine in isolated yeast vacuoles. European Journal of Biochemistry 54:81–91
    [Google Scholar]
  2. Borst-Pauwels G.W.F.H. 1981; Ion transport in yeast. Biochimica et biophysica acta 650:88–127
    [Google Scholar]
  3. Borst-Pauwels G.W.F.H., Theuvenet A.P.R. 1984; Apparent saturation kinetics of divalent cation uptake in yeast caused by a reduction in the surface potential. Biochimica et biophysica acta 771:171–176
    [Google Scholar]
  4. Chlebowski J.F., Coleman J.E. 1977; Zinc and its role in enzymes. In Microorganisms and Minerals, pp 1–140 Weinberg E. D. Edited by New York: Marcel Dekker;
    [Google Scholar]
  5. Durr M., Boller T., Wiemken A. 1975; Polybase induced lysis of yeast spheroplasts. A new gentle method for preparation of vacuoles. Archives of Microbiology 105:319–327
    [Google Scholar]
  6. Eilam Y., Lavi H., Grossowicz N. 1985; Cytoplasmic Ca2+ homeostasis maintained by a vacuolar Ca2+ transport system in the yeast Saccharomyces cerevisiae. Journal of General Microbiology 131:623–629
    [Google Scholar]
  7. Failla M.L. 1977; Zinc: functions and transport in microorganisms. In Microorganisms and Minerals, pp 151–214 Weinberg E. D. Edited by New York: Marcel Dekker;
    [Google Scholar]
  8. Failla M.L., Weinberg E.D. 1977; Cyclic accumulation of zinc by Candida utilis during growth in batch culture. Journal of General Microbiology 99:85–97
    [Google Scholar]
  9. Failla M.L., Benedict C.D., Weinberg D. 1976; Accumulation and storage of Zn2+ by Candida utilis. Journal of General Microbiology 94:23–36
    [Google Scholar]
  10. Fuhrmann G.-F., Rothstein A. 1968; The transport of Zn2+, Co2+ and Ni2+ into yeast cells. Biochimica et biophysica acta 463:325–330
    [Google Scholar]
  11. Gadd G.M. 1981; Mechanisms implicated in the ecological success of polymorphic fungi in metal-polluted habitats. Environmental Technology Letters 2:531–536
    [Google Scholar]
  12. Gadd G.M. 1986; Fungal responses towards heavy metals. In Microbes in Extreme Environments, pp 83–110 Codd G. A., Herbert R. A. Edited by London: Academic Press;
    [Google Scholar]
  13. Gadd G.M., Mowll J.L. 1983; The relationship between cadmium uptake, potassium release and viability in Saccharomyces cerevisiae. FEMS Microbiology tetters 16:45–48
    [Google Scholar]
  14. Gadd G.M., Mowll J.L. 1985; Copper uptake by yeast-like cells, hyphae and chlamydospores of Aureobasidium pullulans. Experimental Mycology 9:230–240
    [Google Scholar]
  15. Huber-Walchli V., Wiemken A. 1979; Differential extraction of soluble pools from cytosol and the vacuoles of yeast (Candida utilis) using DEAE-dextran. Archives of Microbiology 120:141–149
    [Google Scholar]
  16. Kessels B.G.F., Belde P.J.M., Borst-Pauwels G.W.F.H. 1985; Protection of Saccharomyces cerevisiae against Cd2+ toxicity by Ca2+ . Journal of General Microbiology 131:2533–2537
    [Google Scholar]
  17. Kuypers G.A.J.O., Roomans G.M. 1979; Mercury-induced loss of K+ from yeast cells investigated by electron probe X-ray microanalysis. Journal of General Microbiology 115:13–18
    [Google Scholar]
  18. Lawford H.G., Pik J.R., Lawford G.R., Williams T., Kligerman A. 1980; Hyperaccumulation of zinc by zinc-depleted Candida utilis grown in chemostat culture. Canadian Journal of Microbiology 26:71–76
    [Google Scholar]
  19. Lichko L.P., Okorokov L.A., Kulaev I.S. 1980; Role of vacuolar ion pool in Saccharomyces carlsbergensis. Potassium efflux from vacuoles is coupled with manganese or magnesium influx. Journal of Bacteriology 144:666–671
    [Google Scholar]
  20. Lichko L.P., Okorokov L.A., Kulaev I.S. 1982; Participation of vacuoles in regulation of levels of K+, Mg2+ and orthophosphate ions in cytoplasm of the yeast Saccharomyces carlsbergensis. Archives of Microbiology 132:289–293
    [Google Scholar]
  21. Matile P.H., Wiemken A. 1976; Interactions between cytoplasm and vacuole. In Transport in Plants, vol. III, Intracellular Interactions and Transport Processes, pp 255–287 Stocking C. R., Heser U. Edited by Berlin: Springer-Verlag;
    [Google Scholar]
  22. Mowll J.L., Gadd G.M. 1983; Zinc uptake and toxicity in the yeasts Sporobolomyces roseus and Saccharomyces cerevisiae. Journal of General Microbiology 129:3421–3425
    [Google Scholar]
  23. Mowll J.L., Gadd G.M. 1984; Cadmium uptake by Aureobasidium pullulans. Journal of General Microbiology 130:279–284
    [Google Scholar]
  24. Norris P.R., Kelly D.P. 1977; Accumulation of cadmium and cobalt by Saccharomyces cerevisiae. Journal of General Microbiology 99:317–324
    [Google Scholar]
  25. Ohsumi Y., Anraku Y. 1981; Active transport of basic amino acids driven by a proton motive force in vacuolar membrane vesicles of Saccharomyces cerevisiae. Journal of Biological Chemistry 256:2079–2082
    [Google Scholar]
  26. Ohsumi Y., Anraku Y. 1983; Calcium transport driven by proton motive force in vacuolar membrane vesicles of Saccharomyces cerevisiae. Journal of Biological Chemistry 258:5614–5617
    [Google Scholar]
  27. Okorokov L.A. 1985; Main mechanisms of ion transport and regulation of ion concentration in the yeast cytoplasm. In Environmental Regulation of Microbial Metabolism, pp 339–349 Kulaev I. S., Dawes E. A., Tempest D. W. Edited by London: Academic Press;
    [Google Scholar]
  28. Okorokov L.A., Lichko L.P., Kadompseva V.M., Kholodenko V.P., Titovsky V.T., Kulaev I.S. 1977; Energy-dependent transport of manganese into yeast cells and distribution of accumulated ions. European Journal of Biochemistry 75:373–377
    [Google Scholar]
  29. Okorokov L.A., Andreeva N.A., Lichko L.P., Valiakhmetov A.Y. 1983a; Transmembrane gradient of K+ ions as an energy source in the yeast Saccharomyces carlsbergensis. Biochemistry International 6:463–472
    [Google Scholar]
  30. Okorokov L.A., Lichko L.P., Andreeva N.A. 1983b; Changes of ATP, polyphosphate and K+ content in Saccharomyces cerevisiae during uptake of Mn2+ and glucose. Biochemistry International 6:481–488
    [Google Scholar]
  31. Okorokov L.A., Kulakovskaya T.V., Lichko L.P., Polorotova E.V. 1985; H+/ion antiport as principal mechanism of transport systems in the vacuolar membrane of the yeast Saccharomyces carlsbergensis. FEBS tetters 192:303–306
    [Google Scholar]
  32. Passow H., Rothstein A. 1960; The binding of mercury by yeast cell in relation to changes in permeability. Journal of General Physiology 43:621–633
    [Google Scholar]
  33. Paton W.H.N., Budd K. 1972; Zinc uptake in Neocosmospora vasinfecta. Journal of General Microbiology 72:173–184
    [Google Scholar]
  34. Ponta H., Broda E. 1970; Mechanismen der Aufname von Zink durch Backerhefe. Planta 95:18–26
    [Google Scholar]
  35. Roomans G.M., Theuvenet A.P.R., Van Den Berg TH.P.R., Borst-Pauwels G.W.F.H. 1979; Kinetics of Ca2+ and Sr2+ uptake by yeast. Effects of pH, cations and phosphate. Biochimica et biophysica acta 551:187–196
    [Google Scholar]
  36. Serrano R. 1980; Effects of ATPase inhibitors on the proton pump of respiratory deficient yeast. European Journal of Biochemistry 105:419–424
    [Google Scholar]
  37. Theuvenet A.P.R., Bindels R.J.M., Van Amelsvoort J.M.N., Borst-Pauwels G.W.F.H., Stols A.L.H. 1983; Interaction of ethidium bromide with yeast cells investigated by electron probe X-ray microanalysis. Journal of Membrane Biology 73:131–136
    [Google Scholar]
  38. Trevors J.T., Stratton G.W., Gadd G.M. 1986; Cadmium transport, resistance and toxicity in algae, bacteria and fungi. Canadian Journal of Microbiology 32:447–464
    [Google Scholar]
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