DNA:DNA Hybridization Studies on the Pink-pigmented Facultative Methylotrophs Free

Abstract

The genomic relatedness among 36 strains of pink-pigmented facultatively methylotrophic bacteria (PPFMs) was estimated by determination of DNA base composition and by DNA:DNA hybridization studies. A reproducible hybridization system was developed for the rapid analysis of multiple DNA samples. Results indicated that the PPFMs comprise four major and several minor homology groups, and that they should remain grouped in a single genus, .

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1987-03-01
2024-03-28
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References

  1. Anthony C. 1981; Electron transport in methylotro- phic bacteria. In Microbial Growth on Cl Compounds pp. 220–230 Dalton H. Edited by London: Heyden;
    [Google Scholar]
  2. Austin B., Goodfellow M. 1979; Pseudomonas mesophilica. A new species of pink bacteria isolated from leaf surfaces. International Journal of Systematic Bacteriology 29:373–378
    [Google Scholar]
  3. Brenner D.J., Fanning G.R., Johnson K.E., Citarella R.V. 1969; Polynucleotide sequence relationships among members of Enterobacteriaceae. Journal of Bacteriology 98:637–650
    [Google Scholar]
  4. Burton K. 1968; Determination of DNA concentration with diphenylamine. Methods in Enzymology 12:163–166
    [Google Scholar]
  5. Chandra T.S., Shethna Y.I. 1975; Isolation and characterisation of some new oxalate decomposing bacteria. Antonie van Leeuwenhoek 41:101–111
    [Google Scholar]
  6. Colby J., Zatman L.J. 1973; Trimethylamine metabolism in obligate and facultative methylotrophs. Biochemical Journal 132:101–112
    [Google Scholar]
  7. Deley J., Tijtgat R. 1970; Evaluation of membrane filter methods for DNA-DNA hybridization. Antonie van Leeuwenhoek 36:461–474
    [Google Scholar]
  8. De ley J., Tijtgat R., De smedt J., Michiels M. 1973; Thermal stability of DNA:DNA hybrids within the genus Agrobacterium. Journal of General Microbiology 78:241–252
    [Google Scholar]
  9. Denhardt D.T. 1966; A membrane filter technique for the detection of complementary DNA. Biochemical and Biophysical Research Communications 23:641–646
    [Google Scholar]
  10. Den dooren de jong L.E. 1927; Uber protamino- phage Bakterien. Zentralblattfur Bakteriologie, Para- sitenkunde, Infektionskrankheiten und Hygiene 71:193–232
    [Google Scholar]
  11. Eckersley K., Dow C.S. 1980; Rhodopseudo- monas blastica sp nov.: a member of the Rhodospiril- laceae. Journal of General Microbiology 119:465–473
    [Google Scholar]
  12. Gillespie D., Spiegelman S. 1965; A quantitative assay for DNA-RNA hybrids with DNA immobilised on a membrane. Journal of Molecular Biology 12:829–842
    [Google Scholar]
  13. Graf W., Bauer L. 1973; Red bacterial growth in tap water systems. Zentralblatt fur Bakteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene 236:513–530
    [Google Scholar]
  14. Green P.N., Bousfield I.J. 1982; A taxonomic study of some Gram-negative facultatively methylo- trophic bacteria. Journal of General Microbiology 128:623–628
    [Google Scholar]
  15. Green P.N., Bousfield I.J. 1983; Emendation of Methylobacterium Patt et al., 1976; Methylobacterium rhodos (Heumann, 1962); Methylobacterium radiora (Ito & Iizuka, 1971); Methylobacterium mesophilica (Austin & Goodfellow, 1979). International Journal of Systematic Bacteriology 33:875–877
    [Google Scholar]
  16. Green P.N, Gibson D.M. 1984; Carbohydrate metabolism in some methylotrophic bacteria. FEMS Microbiology Letters 23:31–34
    [Google Scholar]
  17. Hanson R.S. 1980; Ecology and diversity of methylotrophic organisms. Advances in Applied Microbiology 26:3–39
    [Google Scholar]
  18. Hayward A.C. 1960; Relationship between Prot- aminobacter ruber and some red pigmented pseudomonads. Journal of Applied Bacteriology 23:ii
    [Google Scholar]
  19. Heumann W. 1962; Die Methodik der Kreuzung stembildener Bakterien. Biologisches Zentralblatt 81:341–354
    [Google Scholar]
  20. Ito H., Iizuka H. 1971; Part XIII Taxonomic studies on a radio-resistant Pseudomonas. Agricultural and Biological Chemistry 35:1566–1571
    [Google Scholar]
  21. Janota-Bassalik L., Pedyk D. 1961; Ability of Flavobacterium extorquens (Bassalik) to utilise various sources of carbon with particular reference to glucose. Acta microbiologica polonica 10:225–238
    [Google Scholar]
  22. Johnson J.L. 1973; Use of nucleic acid homologies in the taxonomy of anaerobic bacteria. International Journal of Systematic Bacteriology 23:308–315
    [Google Scholar]
  23. Johnson P.A., Quayle J.R. 1964; Microbial growth on Cl compounds. 6. Oxidation of methanol, formaldehyde and formate by methanol grown Pseudomonas AM 1. Biochemical Journal 93:281–290
    [Google Scholar]
  24. Kirby K.S., Fox-Carter E., Guest M. 1967; Isolation of deoxyribonucleic acid and ribosomal ribonucleic acid from bacteria. Biochemical Journal 104:258–262
    [Google Scholar]
  25. Kouno K., Ozaki A. 1975; Distribution and identification of methanol utilising bacteria. In Microbial Growth on Cl Compounds. Edited by Terui G. et al. Japan: Society of Fermentation Technology.;
    [Google Scholar]
  26. Legault-Demare J., Desseaux B., Heyman T., Seror S., Ross G.P. 1967; Studies on hybrid molecules of nucleic acids. I. DNA-DNA hybrids on nitrocellulose filters. Biochemical and Biophysical Research Communications 28:550–557
    [Google Scholar]
  27. Mandel M., Schildkraut C.L., Marmur J. 1968a; Use of CsCl density gradient analysis for determining the G-C content of DNA. Methods in Enzymology 12B:108
    [Google Scholar]
  28. Mandel M., Schildkraut C.L., Marmur J. 1968b; Use of UV absorbance-temperature profile for determining G-C content of DNA. Methods in Enzymology 12B:109
    [Google Scholar]
  29. Marmur J., Doty P. 1962; Determination of the base composition of DNA from its thermal denatur- ation temperature. Journal of Molecular Biology 5:109–118
    [Google Scholar]
  30. Mcconaughy B.L., Laird C.D., Mccarthy B.J. 1969; Nucleic acid reassociation in formamide. Biochemistry 8:3289–3295
    [Google Scholar]
  31. Patt T.E., Cole G.C., Hanson R.S. 1976; Methylobacterium, a new genus of facultatively methylotrophic bacteria. International Journal of Systematic Bacteriology 26:226–229
    [Google Scholar]
  32. Peel D., Quayle J.R. 1961; Microbial growth on Cl compounds. 1. Isolation and characterisation of Pseudomonas AMI. Biochemical Journal 81:465–469
    [Google Scholar]
  33. Rigby P.W.J., Dieckmann M., Rhodes C., Berg P. 1977; Labelling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. Journal of Molecular Biology 113:237–251
    [Google Scholar]
  34. Rock J.S., Goldberg I., Ben-Bassat A., Mateles R.I. 1976; Isolation and characterisation of two methanol-utilising bacteria. Agricultural and Biological Chemistry 40:2129–2135
    [Google Scholar]
  35. Schleifer K.H., Stackebrandt E. 1983; Molecular systematics of prokaryotes. Annual Review of Microbiology 37:143–187
    [Google Scholar]
  36. Sneath P.H.A., Sokal R.R. 1973 Numerical Taxonomy: The Principles and Practice of Numerical Classification San Francisco: W. H. Freeman;
    [Google Scholar]
  37. Stackebrandt E., Kandler O. 1979; Taxonomy of the genus Cellulomonas based on phenotypic characters and DNA-DNA homology and proposal of seven neotype strains. International Journal of Systematic Bacteriology 29:273–282
    [Google Scholar]
  38. Stocks P.K., Mccleskey C.S. 1964; Identity of the pink-pigmented methanol oxidising bacteria as Vibrio extorquens. Journal of Bacteriology 88:1065–1070
    [Google Scholar]
  39. Urakami T., Komagata K. 1979; Cellular fatty acid composition and coenzyme Q system in Gramnegative methanol utilising bacteria. Journal of General and Applied Microbiology 25:343–360
    [Google Scholar]
  40. Urakami T., Komagata K. 1981; Electrophoretic comparison of enzymes in the Gram-negative methanol utilising bacteria. Journal of General and Applied Microbiology 27:381–403
    [Google Scholar]
  41. Whittenbury R., Dow C.S. 1977; Morphogenesis and differentiation in Rhodomicrobium vannielii and other budding and prosthecate bacteria. Bacteriological Reviews 41:754–808
    [Google Scholar]
  42. Whittenbury R., Philips K.C., Wilkinson J.F. 1970; Enrichment, isolation and some properties of methane-utilizing bacteria. Journal of General Microbiology 61:205–218
    [Google Scholar]
  43. Wolfrum T., Gruner G., Stolp H. 1986; Nucleic acid hybridisation of pink-pigmented facultative methylotrophs and pseudomonads. International Journal of Systematic Bacteriology 36:24–28
    [Google Scholar]
  44. Zatman L.J. 1981; A search for patterns in methylotrophic pathways. In Microbial Growth on Cl Compounds pp. 42–54 Dalton H. Edited by London: Heyden;
    [Google Scholar]
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