RT Journal Article SR Electronic(1) A1 Lindgren, Viveka A1 Wretlind, BengtYR 1987 T1 Characterization of a Pseudomonas aeruginosa Transposon Insertion Mutant with Defective Release of Exoenzymes JF Microbiology, VO 133 IS 3 SP 675 OP 681 DO https://doi.org/10.1099/00221287-133-3-675 PB Microbiology Society, SN 1465-2080, AB A Pseudomonas aeruginosa transposon insertion mutant with defective release of several exoenzymes has been characterized. The Tn5-751 insertion mutation was located in the previously described xcp-1 locus at 0 min on the chromosomal map and caused several exoenzymes to remain in cell-bound form. At least one of the exoenzymes, elastase, was accumulated in the periplasmic space. The periplasmic elastase had the same M r as the extracellular enzyme produced by the wild-type strain. The virulence of the mutant was comparable to that of wild-type strains in experimental burn infection in mice. The presence of an easily selectable antibiotic resistance marker in the xcp-1 locus offers the possibility of cloning the gene(s) involved in exoprotein secretion., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-133-3-675